- Molecular Tool 'Cirobu.Bra>MRFbΔCH (Izzi 2013)' >
- Region 'REG00000063'
Cis-regulatory Region
Name
Ci-Sna -650/87 E6/E5 mutated
Short name
n/a
Region ID
REG00000063
Status
Curated
Origin
engineered region from C. robusta formely Ciona int. type A
Type of Activity
regulatory_region
(extended_promoter)
Author
Albert Erives (2006-10-01)
Annotator
Patrick Lemaire (2007-11-06)
Curator
Delphine Dauga (2011-11-14)
The region extends from -650bp to +87 bp of the start of cDNA AF002987. Mutation of the E5 and E6 sites to TT core E-box sites leads to a decrease in the activity of the region.
This region is named Ci-sna AC-5 in the paper (Erives et al 1998).
Modification of Ci-Sna -650/87 by substitution
- Ci-Sna -3200/codon 15 REG00000051 [ regulatory_region (extended_promoter) ]
- Ci-Sna -3200/codon 15 delta 262bp REG00000052 [ regulatory_region (extended_promoter) ]
- Ci-Sna -3000/-68 REG00000053 [ regulatory_region (complex_region) ]
- Ci-Sna -2200/codon 15 REG00000054 [ regulatory_region (extended_promoter) ]
- Ci-Sna -1700/-157 REG00000055 [ regulatory_region (complex_region) ]
- Ci-Sna -650/87 REG00000058 [ regulatory_region (extended_promoter) ]
- Ci-Sna -650/87 E7 mutated REG00000059 [ regulatory_region (extended_promoter) ]
- Ci-Sna -650/87 E7/E6 mutated REG00000060 [ regulatory_region (extended_promoter) ]
- Ci-Sna -650/87 E7/E6/E5 mutated REG00000061 [ regulatory_region (extended_promoter) ]
- Ci-Sna -650/87 E6 mutated REG00000062 [ regulatory_region (extended_promoter) ]
- Ci-Sna -650/87 E6/E5 mutated REG00000063 [ regulatory_region (extended_promoter) ]
- Ci-Sna -650/87 E5 mutated REG00000064 [ regulatory_region (extended_promoter) ]
- Ci-Sna -444/87 REG00000069 [ regulatory_region (extended_promoter) ]
- Ci-Sna -672/-168 REG00000065 [ regulatory_region (enhancer) ]
- Ci-Sna -618/-356 REG00000066 [ regulatory_region (enhancer) ]
- Ci-Sna -3200/codon 15 delta 1.5kb REG00000214 [ regulatory_region (extended_promoter) ]
5,678,6335,679,369
| Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment | |
|---|---|---|---|---|---|
| 1 | bHLH | n/a | CAACTG | [5,678,683 / 5,678,688] | |
| 2 | T-Box | n/a | GATCCCACCT | [5,678,737 / 5,678,746] | |
| 3 | T-Box | n/a | TTTGGCACCT | [5,678,873 / 5,678,882] | |
| 4 | bHLH | n/a | CACCTG | [5,678,929 / 5,678,934] | |
| 5 | bHLH | n/a | CATATG | [5,678,951 / 5,678,956] | |
| 6 | bHLH | n/a | CATTTG | [5,679,045 / 5,679,050] | |
| 7 | bHLH | n/a | CAAATG | [5,679,075 / 5,679,080] |
Aniseed Coordinates: [5,678,633 / 5,679,369] on scaffold KhC3
JGI version 1.0 version of the sequence, in which site E5 and E6 were mutated from CAACTG to a TT core E-box site: CATTTG. Note the E5 site in not present in the JGI assembly.
Show sequence
Length: 737
CAGAAGATCGGTGTGTTTTGCTGGACAAATATTGAGAAAATCTGTGGCGACAACTGTGGT
CTGATTTACAGGCGAGTTGGACGGAGTTGACAGGTCGCGAAAGCGATCCCACCTTCGTGT
CTGGGTTTCGAGGTGCCGCCCGTCGTGCAGTAGCATTTGTGCGGTCAAGTTGAGAGCAAG
AAGACACTACAGCGCGCTTTGAAGCGTCCAAGCCGCCCGCTGAGCAGGGCGGATAGCTCG
TTTGGCACCTCGTCTGGCATCGTCCGCACTGTCAACCTTCTTCGTACAAACGTCCTCACC
TGCCGCGCAGGATTGCTTCATATGGCAGACCGAGTTGAGTGGGAAATCATATTCTGGTTG
CTTCGCTTATAGAGCTAGCTCACTAGAGACTAGAGTGTTGTGAATGATGAATCATTTGAA
TTAAGCATTTTGTTTAGTTGATCAAATGAGTTTCAGTTTGATGTTCAGGACTGCTAAGTA
ATGCAGTGAAAGGAATTCTAAAGTGATTATATTTAGAAATGGGCTATCAGTGCTTGATTA
CTGTTGCAGGGTTATTTAAAAATGCAGTCGAACAGTCTAAGAAAGCTTAGCTATATTTGA
AGGATTATGTATACTTATTTCATTAGAGCAATATTTTTTTTTTTATTTTAGACTTTTGGT
ACATATTCAAAATATCAAAATTCTCACTGTGAAATATATTACTGTGATTACATCATGACC
TCCGTCGAGCCCATGCT
