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  1. Region 'REG00000065'

Cis-regulatory Region

Name

Ci-Sna -672/-168

Short name

n/a

Region ID

REG00000065

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Albert Erives (2006-10-01)

Annotator

Patrick Lemaire (2007-11-06)

Curator

Delphine Dauga (2010-02-26)


Description

The sequence below goes from -672bp and -168bp upstream of the start of cDNA AF002987. It recapitulates ci-san expression in the B4.1 mesodermal lineages (except the TVCs).

This region is named 504bp (syn and anti for the two constructs) in the paper (Erives et al 1998).

Hierarchy of Regulatory Regions

Modification of Ci-Sna -3200/codon 15 by deletion

Overview of functional TF binding sites in this region

5,678,6115,679,114

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 bHLH n/a CAACTG [5,678,683 / 5,678,688]
2 T-Box n/a GATCCCACCT [5,678,737 / 5,678,746]
3 bHLH n/a CAACTG [5,678,786 / 5,678,791]
4 T-Box n/a TTTGGCACCT [5,678,873 / 5,678,882]
5 bHLH n/a CACCTG [5,678,929 / 5,678,934]
6 bHLH n/a CATATG [5,678,951 / 5,678,956]
7 bHLH n/a CATTTG [5,679,045 / 5,679,050]
8 bHLH n/a CAAATG [5,679,075 / 5,679,080]

Sequence

Aniseed Coordinates: [5,678,611 / 5,679,114] on scaffold KhC3

JGI version 1.0 of this region. site E5 is absent.

Show sequence

Length: 504


AATTGTATTTACCATTATAAAACAGAAGATCGGTGTGTTTTGCTGGACAAATATTGAGAA
AATCTGTGGCGACAACTGTGGTCTGATTTACAGGCGAGTTGGACGGAGTTGACAGGTCGC
GAAAGCGATCCCACCTTCGTGTCTGGGTTTCGAGGTGCCGCCCGTCGTGCAGTAGCAACT
G
TGCGGTCAAGTTGAGAGCAAGAAGACACTACAGCGCGCTTTGAAGCGTCCAAGCCGCCC
GCTGAGCAGGGCGGATAGCTCGTTTGGCACCTCGTCTGGCATCGTCCGCACTGTCAACCT
TCTTCGTACAAACGTCCTCACCTGCCGCGCAGGATTGCTTCATATGGCAGACCGAGTTGA
GTGGGAAATCATATTCTGGTTGCTTCGCTTATAGAGCTAGCTCACTAGAGACTAGAGTGT
TGTGAATGATGAATCATTTGAATTAAGCATTTTGTTTAGTTGATCAAATGAGTTTCAGTT
TGATGTTCAGGACTGCTAAGTAAT





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