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CIS-REGULATION

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  1. Region 'REG00000068'

Cis-regulatory Region

Name

Ci-Sna -618/-360 T2/T3 mutated

Short name

n/a

Region ID

REG00000068

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Levine Team (2006-10-01)

Annotator

Patrick Lemaire (2007-11-06)

Curator

Delphine Dauga (2010-02-26)

View position in WashU

Description

Mutated version of ci-sna -618 -360 in which the two T-box sites are inactivated by point mutation (all the G were mutated in A).


This region is named 262bp (G=>A)2 in the paper (Erives et al 2000).

Hierarchy of Regulatory Regions

Modification of Ci-Sna -618/-356 by substitution

Regulated gene(s)

KH2012:KH.C3.751 (SNAI1; SNAI2; SNAI3)

Construct(s) made to test this region

CONS00000058: pCi-Sna -618 -360 T2/T3 G->A -fkh::NLS-LacZ

Show in situ data
Overview of functional TF binding sites in this region

5,678,6715,678,928

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 bHLH n/a CAACTG [5,678,683 / 5,678,688]
2 bHLH n/a CAACTG [5,678,785 / 5,678,790]
3 bHLH n/a CAACTG [5,678,901 / 5,678,906]

Sequence

Aniseed Coordinates: [5,678,671 / 5,678,928] on scaffold KhC3

Sites T2 (TTTGGCACCT) and T3 (GATCCCACCT) were respectively mutated into TTTGGtACCT and GATCCtACCT. Sequence derived from AF302999, which differs slightly from the JGI v1.0 sequence.

Show sequence

Length: 262


AATCTGTGGCGACAACTGTGGTCTGATTTACAGGCGAGTTGGACGGAGTTGACAGGTCGC
GAAAGCGATCCTACCTTCGTGTCTGGGTTTCGAGGTGCCGCCCGTCGTGCAGTACAACTG
TGCGGTCAAGTTGAGAGCAAGAAGACACCACAGCGCGCTTTGAAGCGTCCAAGCCCGCCC
GCTGAGCAGGGCGGATAGCTCGTTTGGTACCTCGTCCTGGCATCGTCCCGCAACTGTCAA
CCTTCTTCGTTACAAACGTCCT

Reference(s)

Erives et al. (2000). Characterization of a maternal T-Box gene in Ciona intestinalis. Dev. Biol. 225(1):169-78

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