A 3 kb region of Ci-trop 5-flanking sequence was characterized via electroporation of lacZ fusion genes into fertilized Ciona eggs.

-A minimal, 114 bp enhancer (between -1,4 kb and -1,3 kb) was identified that is sufficient to direct the expression of a heterologous promoter in the notochord. DNA binding assay indicate that this enhancer contains two sets of low affinity Brachyury half-sites, which are bound in vitro by a GST/Ci-bra fusion protein.

-The region between -1,3 kb to -1 bp drives expression in the tail epidermis and in the trunk mesenchyme that is normally silenced by negative control elements between -1,4 kb and -1,3 kb.

Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.

Coordinates in the paper : -1400bp/-1285bp

Modification of Ci-Trop -1393/6 by deletion

KH2012:KH.C7.260 (TPM1; TPM3; TPM4)

CONS00000174: pTrop -1393/-1278::LacZ

4,512,9774,513,091

Aniseed Coordinates: [4,512,977 / 4,513,091] on scaffold KhC7

This sequence was found using a blast on the JGI site with the fragment of sequence given in the publication "Regulation of Ci-tropomyosin-like, a Brachyury target gene in the ascidian Ciona intestinalis" Di Gregorio A, Levine M. Development. 1999 Dec;126(24):5599-609.

Di Gregorio et al. (1999). Regulation of Ci-tropomyosin-like, a Brachyury target gene in the ascidian, Ciona intestinalis. Development 126(24):5599-609