Description

The minimal cardiac enhancer for the ciona Mesp gene demonstrates direct activation by the T-box transcription factor Tbx6c. Inactivation of site B by mutation kills the activity of this region.



JGI transcript model was taken as reference for the region s coordinates (same in the paper).

Hierarchy of Regulatory Regions

Modification of Ci-Mesp -110/39 by substitution

Regulated gene(s)

KH2012:KH.C3.100 (MESP2; MSGN1; PTF1A)

Construct(s) made to test this region

CONS00000204: pCi-Mesp -110/39 T-boxB-mutated1 pfkh::lacZ

Overview of functional TF binding sites in this region

3,537,1593,537,301

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment
1	T-Box	n/a	CGGCGATAA	[-82 / -74]
2	T-Box	n/a	TAGACACCT	[-63 / -55]

Sequence

Aniseed Coordinates: [3,537,159 / 3,537,301] on scaffold KhC3

The sequence was retrieved from the JGI v1.0 assembly using the sequence found in the paper. T-box site B, AGGCTA, was inactivated by mutation to AGTCAA.

Reference(s)

Davidson et al. (2005). Uncoupling heart cell specification and migration in the simple chordate Ciona intestinalis. Development 132(21):4811-8