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CIS-REGULATION

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  1. Clone 'cicl027b03' > 
  2. Region 'REG00000777'

Cis-regulatory Region

Name

Ci-Nut1 -1030/94

Short name

n/a

Region ID

REG00000777

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Kotaro Shimai (2010-06-29)

Annotator

Clara Degos (2011-08-10)

Curator

Delphine Dauga (2011-08-10)

View position in WashU

Description

Region upstream of Ci-Nut1 from -1030bp to +94bp, named -1026 in the paper. This region can drive expression in neural tube.

Three putative regulatory motifs were present in this region : 2 ZicL binding sites and one Fox binding site.


Transcription start site is based on the start of the first exon of the KH transcript model.

Be carefull the coordinates in the paper may be different from ANISEED coordinates.

Hierarchy of Regulatory Regions

Modification of Ci-Nut1 -1119/94 by deletion

Regulated gene(s)

KH2012:KH.C14.4 (ADRB2; P2RY12; RGR)

Construct(s) made to test this region

CONS00000740: pCi-Nut1 -1030/94 pVenus::EGFP

Show in situ data
Overview of functional TF binding sites in this region

1,413,7941,414,918

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 ZnF_(C2H2) n/a catgctgtg [-74 / -66]
2 Fox n/a tgtattgttt [-63 / -54]
3 ZnF_(C2H2) n/a gtcgctttg [-41 / -33]

Sequence

Aniseed Coordinates: [1,413,794 / 1,414,918] on scaffold KhC14

Transcription start site is based on the start of the first exon of the KH transcript model.

Show sequence

Length: 1,124


ACCCTCCAATAACAGGGTAAGATGCATGCGAAAAAAACGATTGATTTTGTGCATATAAAA
TATTTATAATAGTCTGGGGTTATGTGAGTGAAATAGCTGGTTATATCTAACTGCAGCCGG
AAATACGTGCTCTTTGCAGAAAGGAAAATCCATTTCTCGTGTATTGCAACATACGGTATA
GTAGGTTTAGATGGGTCACCTTTACCACAAATAACATCCAAATATCCTGAGCATGTTTTA
AACATTTAACAACGGTATATGTGGAAGTTGCGAGAATACGGTTTGATAATTCATTAAATA
TGCTTTGTTTACTACAAAATGGGACGGGAAAATAAAATAAAAAGGTGTCCTATCTTCCCC
CATCCTACTATATATATCATATTTTCTAATCCTGTTTTAGCAATTAACAACGCTCGTTTA
AAGTCGTGGGTATATGCGGTTACATAAATTTAAAAATATTATTTGTTTACTTCAAAATAG
AACGATAAAAGCGTGTATAAACATGTTGTATCATCTTACCCCATCCTGCTACAAATATAA
ACAATTTCATGATAAGAAAATGCACATGTTTGTTATGCCGCGTGTTGTGTACAGTTCTGG
TTAGGATTTCAGAACACACACACAGAAAATCGGTCGTGCCTGCTGCTGCAACTAGTGCGA
CACCTCAATAATTATGAGACTGCCTCAGTGCATTCCTATCAACGTGTTTTGCGCTCGTAT
CACCCAAAAAGCGTGCCTCGTGCTGCCTACACGGTGTGGCTACATAAAAACGTCGCGTGT
TTTACTGTTCCAGTTCGTTTTTGTATTCGTGGAAATAGGTTCGCATTTTTTATTAAGAAA
GAGTTTGGTTTTCGAGGTCACTGAATTGCAATTAGAAGGCATTTAATAGTAGCACTAGGA
CACTCTATTCACTGCGTTGTCTAAATCCATGCGAAACAACAAAATAAGTCGCAAGACATG
CTGTGCGTGTATTGTTTTTTTGAAACCCCGTCGCTTTGTGAAAATCTGGTTGATTATTTT
TTCGTACCAGTTTTACAGTTTAAATACGACTGTGCTTCAGTTTTTGTTAGTATTGAGTTG
TACACTATATCAACAACATGGAAATTGATTTTGGATTTGCAGAA

Reference(s)

Shimai et al. (2010). Upstream regulatory sequences required for specific gene expression in the ascidian neural tube. Zool. Sci. 27(2):76-83

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