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CIS-REGULATION

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  1. Clone 'AHC0AAA75YK10' > 
  2. Region 'REG00000815'

Cis-regulatory Region

Name

Ci-Noto1 -267/41

Short name

n/a

Region ID

REG00000815

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Hiroki Takahashi (2010-07-02)

Annotator

Clara Degos (2010-07-22)

Curator

Delphine Dauga (2010-07-26)

View position in WashU

Description

Region upstream of Ci-Noto1 from -267bp to +41bp. This region cannot drive Notochord expression.


The corresponding construct is named "p(-0.3k)Noto1/lacZ" in the paper.

Be careful, coordinates in the paper may be different from ANISEED coordinates. We took the KH transcript model as reference.

Hierarchy of Regulatory Regions

Modification of Ci-Noto1 -407/41 by deletion

Regulated gene(s)

KH2012:KH.L20.18 (KH.L20.18)

Construct(s) made to test this region

CONS00000778: pCi-Noto1 -267/41::LacZ

Show in situ data
Overview of functional TF binding sites in this region

513,873514,180

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [513,873 / 514,180] on scaffold KhL20

Coordinates are based on the first exon of the KH transcript.

Show sequence

Length: 308

ATGATACGAATATATATTTCTGTAAAGATTTTCTTGTTTACTATTAAATGGGTCGAAAAA
AGAGAACGAAAAACTGACCCATCTTACCCCAACCTGATAGATATTTCTTTGTTTGTTCTT
TTTACATCCAGGCAAGTAACGCTGTAGTGTAAAAGTCGTTTAAAAAAAATAAACAAAGAA
ATATCTACGCTAAAAACGCTGGGTGTATATAGATATGATGGGCAACAAAACCCAATCAAA
TACGTTCATTTAAAATGTTCTTAGTAGACAGGTAAACTATTACAAATAATCCAAAATTAT
GAAGTTGT

Reference(s)

Takahashi et al. (2010). Regulation of notochord-specific expression of Ci-Bra downstream genes in Ciona intestinalis embryos. Zool. Sci. 27(2):110-8

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