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CIS-REGULATION

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  1. Region 'REG00000823' > 
  2. Transcript 'Boschl.CG.Botznik201...' > 
  3. Region 'REG00000847' > 
  4. Region 'REG00000814' > 
  5. Region 'REG00000835'

Cis-regulatory Region

Name

Ci-tensin 2942/6022

Short name

n/a

Region ID

REG00000835

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Hiroki Takahashi (2010-07-07)

Annotator

Clara Degos (2010-07-19)

Curator

Delphine Dauga (2010-07-26)

View position in WashU

Description

Region of Ci-tensin from +2942bp to +6022bp. This region drives notochord expression.

The corresponding construct is named p(-3.0 k)tensin/lacZ in the paper.
Be careful, coordinates in the paper may be different from ANISEED coordinates. We took the KH transcript model as reference.

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.C9.28 (TNS1; TNS2; TNS3)

Construct(s) made to test this region

CONS00000798: pCi-tensin 2942/6022::LacZ

Show in situ data
Overview of functional TF binding sites in this region

4,280,3614,283,440

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [4,280,361 / 4,283,440] on scaffold KhC9

Sequence found thanks to the primers used in the paper : Ci-tensin (BamH-R) CGGGATCCATTGCTCTGTGTTAAAATATTC and Ci-tensin (-3.0 kXba-F) GCTCTAGAGCCATGACGACCCAAGCATGT.

Show sequence

Length: 3,080

TATCCAAGCCAATGACGACCCAAGCATGTGCCTCATGCACACTGACGTAATCTTCTTTCT
CCATCCTTGGTACCACGTTTCACCTCAAACGAGTCCATCAACTAGTTAACTAAAGTGCAA
TTGGTTTGATTTCCCATTTACTGCCGTGTTGATCCCCGCTTCCGACGTAAAAGTTACCGT
CTCGGTTCGACGCAGAGTGCTGGCCTCAAATGTCCCGACAGTAAAAGATAAAACGGGAGT
TGGGTCGTTTAACTGTGAGAACGACGGCCGCTGTGTTTTGACGACAAGCGAGCAGGTAGT
TGGACGGATGAGCCGAGGTACGTCACAGTTTAGTCACTCTAGTTAAGCCCATGTACAGGT
TGTGTTCAAAGAGTTTTGCCTTTAGATCTCATTGTACCTAGTTTAGTTTTTAAACAAGCT
GAGAAAGCGTTATCATCGAATAGTGGCCAGGAGTTAACAAAGAAAGGGAGCGAATTTATT
TACTGTCAAGTTTGACCTTCGCAAAGCAGGGGCAGGGACTTCATTTCTGTCTGCTGATAA
GTGGCCCTGATCCGTTCGCTCTATTGTTTATGACAGTCGAAGTCAAATGAGTCTGCACTT
TTACCATGGCCGTTTCGAATTTTTCGATCCATGAAGCCCGCTTAAACCTGTTACCACTGA
CAGCACACTTGGCTGTATTGGGCTCGACAAACCATTGCCACTTGACACCACTCAACGAGC
CTTTTGCCACGAGGGCAATCAATACATGTTTATCCGCGCTTTGTAACTGTATTGTATGAT
GCAAGGTTGCAGTGCAGGCTAATGGCTAATTAAACCAGCGAAGTAGAAGAGAAGTTTGAA
TAATTTTGCAATTTATCGCGGAAAAAAACTAAATCGGGAAGTTTTGCATATAAAGCTCAG
TCGGTGCGCGTAGCACCGTCGCTTTATGTTGTATAGATATAGAAACTCATTAGCTTAGAC
GTGCAGCAGGGTTGTGAGGTTTCTGACAACTTTGATGCCAATCGTAGTTGTCTATTTCAG
GCGAAGTGACGTGTCGACGACAATAGATATCTTATTAGCTGTCTATGAACAACCTAGAAA
TTTTATTTGTGGTAATATGCATAGTGTAAGATACCGGTTCCGTAAACGCGTGCCATATAC
CCTAAAGTTAATTGCCAGTACTGTAATAAGGTTGCGCGCAAATGGGGTAAATGTGTATTG
TAGGCTGACCAGTAAATGGTATATGCATTGTGTACATAAGCATTCCACGGTTTAATTAGT
AACGTTACTAATATTTTTACAAACATGGCTTTTATAGTAAGCGTTACCATAGTATTTGAC
GGCAGATTTCGTTATAAGCGCTATACCATAGTATTTGACGGCAGAATTAGTTTAGCATCG
AGTCCTGCTATTTTCGCTCTTGATTTAATCAGGTAAGCAGGAGCCAGGGACACAGAACAA
CAGATGTCGGATACAGGATGTTTGTTAACCAAACCACAGGACGAACCGCACTCTAATCGC
AAAGGATTGTTTCGTCTATCTGCCCATTCAGGCTCGTACGAAAACGAACATTAAATGGTT
GTATATTGATTGTATTACTTTGCGCATTTAGCTTATTTGGAATACGCTTACGCTGCATTG
GACCTGTGTTATAGGGTCGTTCGAGGTCGTACCCTGTGCTCCAATGGTTAGGCATGGAGC
ATGCGCACTAATCGTATTATGACGATATGGCGGTATTCCTTTCACGGTAATTGAATTGGA
AAATGAATAACTCGGTATGACCAATGCTTGTAAAGTTGCGATTATAGCGCGCAACGGTCA
GCGATAGTCGAGTAATGTTTGCCTAAGATTTTCACTTCGGTGTGTTTTCTAGTTTACTGG
GAAATGCGAAGTTACGAGGACAGCATTCGAGTGACTTTGTTTACTCTGGAAATCTGTTGT
TTGCTTCGCAACTATTCAGCTTGCTCGACCGCTTGTTCAAGGCAGAGATTGGCTCGCTAA
TTAATGTTTACCCGCCTGGGCGATGCCTGCCTTTCTCGGGCCGACGGATGGCGTATGAGT
GCGAATCGGCGATTGAAGATCACCGGCCGTCATGTAGGAGTCCAGATCGCCCGCAGAAGT
TCGGTCGCAGGTTCGGCTGTAGGTCAGCGTCGCTGGCGGGTTTACGACCTAGTGTAAAAG
CGGTGCATACGGTCTCGTTGGCCGGACGACTGTAAAACCGCAGCAGTTTAATAAGTTACT
ACTATACTGGCGTCGTATTGCAAGGTTTCCGTCTCTGCGGTTTCAAGGCGAAGAATAATG
CGGGTTTTAGTGTACCCATAGACAGTAAGATCAATTTTGCGGGGTTATAACTGTTTACAT
AAGCAGGGCAGGATAGGAAAGTAATTTAATCAATCGCTTAAAAAAAGCAATGCAAAAGAA
TTTACCAACTATTCGTTTGCTTGGATTAGCTGGTTATTTATTACTTTCTATACGGGCAGC
GTATGCGAGAAACGATGTTGTCTATCAGTGGAAATGCCGGGGAGCCGCGCATGAGGTCAC
ATACCCCCATTGACAATGCTCGGGAATTCCTACTTTGCCGTGGAATTCGGTGTCTTTCAA
GACGCTTTTTACTCGTGTATGTTTAGTATTTGAATTATTTGGGATGGAGTTAAAGCGATC
TGCAGCGTCCATTACAAAGGCTGTATTTCTAGGCGTAAAAAGCCACATAGATCGTCCGCG
AATCCCCGCTACCGGTTAATGTAAAAAACGCCCAGATCTTGGCGTCAGAAGTCCGAACTT
TATATCGCATTTAGCTGCGAAACTACCAGCAATAGTGGATGACAGGAGACGACAGCTTTA
GTGCGATTATAAAAAGCAAATGATTTAAATATCAAATCGCGGCAGAGGGCATTCAAAATC
ATCGCCTTAATTTATACAGCGCCTAGAGTCCGAAAGATCAGAATTTTGTTATTAAAGTTG
TCATTACTTGGATGAGAGCCTAAATTACCACTTGTTGGTTTACAGGTCAGGTCGTTTGTT
GAAAAAGAGCTGTTCTACCATTTGTATTGTTAAAGATCAACGGAAATACGGAATATTTTA
ACACAGAGCAATGAGAGAAG

Reference(s)

Takahashi et al. (2010). Regulation of notochord-specific expression of Ci-Bra downstream genes in Ciona intestinalis embryos. Zool. Sci. 27(2):110-8

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