Description

Region upstream of Ci-Tbx6b from -145bp to -13bp. This region can drive muscle expression from neurula stage.
Region named 112bp Ci-Tbx6b CRM fragment in the paper.
Binding sites are present in this region : one E-box site, one CREB binding site and one T-box site. Here the E-box site was mutated (CAACTG=>ACCAGT). This mutation has no effect on expression.


ANISEED coordinates are different from the coordinates in the paper. Coordinates are based on KH transcript model.

Hierarchy of Regulatory Regions

Modification of Ci-Tbx6b -145/-13 by substitution

Regulated gene(s)

n/a

Construct(s) made to test this region

CONS00000822: pCi-Tbx6b -145/-13 E-box mutated

Overview of functional TF binding sites in this region

26,96027,091

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment
1	T-Box	n/a	TCAGTGTGAC	[-25 / -16]
2	bZIP	n/a	TGACG	[-19 / -15]

Sequence

Aniseed Coordinates: [26,960 / 27,091] on scaffold KhS654

The E-box site was replaced by the mutated sequence (CAACTG=>ACCAGT).

Reference(s)

Erives et al. (2009). Non-homologous structured CRMs from the Ciona genome. J. Comput. Biol. 16(2):369-77

Kugler et al. (2010). Temporal regulation of the muscle gene cascade by Macho1 and Tbx6 transcription factors in Ciona intestinalis. J. Cell. Sci. 123(Pt 14):2453-63