Description

Region upstream of Ci-Tbx6c from -2087bp to -1780bp and able to drive muscle expression. This region is a cis-regulatory module, and named Ci-Tbx6c CRM in the paper.

Aniseed coordinates are different from the coordinates in the paper. We took the KH transcript as model.

Hierarchy of Regulatory Regions

Regulated gene(s)

n/a

Construct(s) made to test this region

CONS00000823: pCi-Tbx6c -2087/-1780 pFoxAa::LacZ

Overview of functional TF binding sites in this region

34,18734,493

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment
1	bHLH	n/a	CAGTTG	[-2,001 / -1,996]
2	T-Box	n/a	ACTCACACTA	[-1,924 / -1,915]
3	ZnF_(C2H2)	n/a	CGTTGGGCTTC	[-1,832 / -1,822]

Sequence

Aniseed Coordinates: [34,187 / 34,493] on scaffold KhS654

Sequence found thanks to the primers used in the paper.

Reference(s)

Kugler et al. (2010). Temporal regulation of the muscle gene cascade by Macho1 and Tbx6 transcription factors in Ciona intestinalis. J. Cell. Sci. 123(Pt 14):2453-63