Description

Region of 230bp from the proximal cis-regulatory region of 2.2kb upstream of Ci-FCol1. This region can drive expression in muscle.

In this region there is four Tbx6 sites, and one Macho1.

Aniseed coordinates are based on the JGI transcript.

Hierarchy of Regulatory Regions

Modification of Ci-FCol1 -2200/1 by deletion

Regulated gene(s)

KH2012:KH.S654.2 (TBX2; TBX3; TBX6)

Construct(s) made to test this region

CONS00000828: pCi-FCol1 -229/1::LacZ

CONS00000826: pCi-FCol1 -229/1 pFoxAa::LacZ

Overview of functional TF binding sites in this region

1,117,2401,117,469

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment
1	ZnF_(C2H2)	n/a	GGCGACCCTTTG	[-185 / -174]
2	T-Box	n/a	ACTGTGTC	[-157 / -150]
3	T-Box	n/a	ACGTGTAAG	[-49 / -41]
4	T-Box	n/a	CGGAGTGCG	[-33 / -25]
5	T-Box	n/a	CACTGTGGA	[-10 / -2]

Sequence

Aniseed Coordinates: [1,117,240 / 1,117,469] on scaffold KhC7

Reference(s)

Kugler et al. (2010). Temporal regulation of the muscle gene cascade by Macho1 and Tbx6 transcription factors in Ciona intestinalis. J. Cell. Sci. 123(Pt 14):2453-63