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CIS-REGULATION

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  1. Region 'REG00000888' > 
  2. Region 'REG00000696' > 
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  4. Region 'REG00000886'
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Cis-regulatory Region

Name

Ci-Pitx -2750/-2250

Short name

n/a

Region ID

REG00000886

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Lionel Christiaen (2011-08-10)

Annotator

Jonathan Luchino (2011-08-10)

Curator

Delphine Dauga (2011-08-10)

View position in WashU

Description

Region from -2760 bp upstream the +1 to the first 140bp of the exon2 is the minimal enhancer which drives the entire Ci-Pitxc expression.


Region from -2750bp to -2550bp is necessary to direct expression in the anterior stomodeal cells.

Hierarchy of Regulatory Regions

Modification of Ci-Pitx -2751/-2540 by deletion

Regulated gene(s)

KH2012:KH.L153.79 (PITX1; PITX2; PITX3)

Construct(s) made to test this region

CONS00000851: pPitx pfkh -2750/-2250::LacZ

CONS00000852: pPitx pdistal -2750/-2250::LacZ

Show in situ data
Overview of functional TF binding sites in this region

00

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [0 / 0] on scaffold

This sequence was found using the sequence upstream the coding sequence of the exon2 of Pitx with a blast on the JGI site.

Show sequence

Length: 500

GAACGGACAATTATTTCTCTTCTGCGGCCGCTGCGGCAGTTGCTGCCCACCCACCGTATC
ATCATTACTTTAACCAACAAACGAACAATTATAACTATAGCCCGTATCACAGTGGGGGCT
ATATTGGGGGCCTGTCCGGTGGGTCAATTTCAGGGGGTTCGCCATCTTCGTCCCCGCCGT
CGAATACCTCACCCCTATTTGGGGGTTCCCCGGGAACCCAAATTGCGCCATCAAGCTCCG
CTACGTCACCGTTTTCGGCTGGTTTGTCGAGGTACGCCGGAAAGTTTTCGGCCGGGTTTT
CGAAGCTCAATTCCGGCGGAAATGGACCTGGAGCCTCGGCCCTTTACCCTGGCAGCGGTT
TGGCTTCGCTTGTGGCTTCTGGAAGTAGTTACTCGCCTCAGAATGAGAAAATGGAACTGC
AGCCGAAATCATCGAGGGTCCCTTCACACGAACAAGTTTCAAAGTCATATTTTGAGCAGC
CTTACTCTAAATGCAAAGAC

Reference(s)

Christiaen et al. (2005). A modular cis-regulatory system controls isoform-specific pitx expression in ascidian stomodaeum. Dev. Biol. 277(2):557-66

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