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CIS-REGULATION

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  1. Region 'REG00000987'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C10:4438567-4440059|Msi

Short name

Musashi 1.5kb intronic / Fragment3 (Awazu2004)

Region ID

REG00000987

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Yasunori Sasakura (2015-06-20)

Annotator

Yasunori Sasakura (2015-06-20)

Curator

Marion Gueroult-Bellone (2022-11-08)

View position in WashU

Description

This 1492bp Musashi enhancer, fused with the TPO promoter was used to drive expression in the endostyle of juveniles.

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.C10.264 (HNRNPDL; MSI1; MSI2)

Construct(s) made to test this region
n/a
Overview of functional TF binding sites in this region

4,438,5674,440,059

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [4,438,567 / 4,440,059] on scaffold KhC10

Show sequence

Length: 1,493

GtgcaggttgaagatgcaatgcTAATGTTCGACAAACAGACGAACCGCCACAGAGGTAAG
CAGTTCATATAATGACATCACATAAATATGACGTCACAAAACTGTGACGTCACAGACTTT
TAACACAAATTATTTTATTATTACAACTTTAAAAGAAAAAATCCGAATTTACTAATTTTA
CAGTGACGTCACTATGGTGCTATTATGACGTAAGTTTCGTGGTAGCTTGTGTGCCGAAGC
AGAACATGTAATAGACAATCCACGTAGATTAGTTTTGTTATTTTACCATAGACCACCTAT
GTTTCCCATACTAAGCAGTAACGTGGACCGTAATTCTGTCAGCTTTTTGTAAAATACAGC
AGTGGTTCTTAACCTTTTTAAACACCAGAATCTTTGTACCTCTATGTACTTACTGTCCAA
TGTTTAAAGTCATTGAAAAAACATTATTTTTTATAGAAAAAATGCATTTTATTATAAAAA
CGTATAGTTTTTTTGGGAAAATGCTCGGATAGAGGTCCGAATTTGGGAGTTCGGCCGAAC
CCAGGTTAAGAACCACTGTTCTAATACTAGCATAAACTAAACCGAGTCTTAGATTTTGTG
CTGTAACTAAACTTGTAAGAAAAAGGAGGTTTTGCTTCCTTTGCAGCGTTTTCGATCCAA
TTTGTTGTGGGTTAATTTGAGTAGTTTTAGCTCACTATGACGTAGTTATGACGTATCTGT
GACGTAGAAGGATGTATAGTACAATGCACGGTGTGAGACGTGGTAGAGTCTAGAGTGATT
ATAGAATATAGTTTCGGTAAAAAACAGTCGCGATTTTCAAACGTTACCCCAAAAACAATT
TCAACGCTCCTATGACGCGTTTATTTAGTGACGTCACGACCGTAGCTTCATACTGTATAT
CGTTGCCAATTCAGGCGATTATTTCGTTACGATTGTATGAAATTGTAAAAGAACAAACGA
CATTGTGGGCAAACGTCACGTAATAAGTAGTTAAAATAACAGCCATTGTTACGATTACAG
CCGTTAAACTCATTGTTCAAGTTATCCCAATGTTTTTGCTGCGCTATTAGCAACCGTCGC
GGTAGCAGTGGTTATGAATACATTCTTATCCCGTTACTAACAGTTTAAACGGTCGCTCTT
ACCGGCACGTTGGTGTATTTAATAAATTTGAGATCCGTTGTAAAGCAAACATGTTTCGAA
AAGGTGGCGTCCGAATGCCCGGATTGTGGCGTCACAATACGTACGGACCCGGATCCATCG
AAACAGTATTAAGCACTTCTGTGATTTAGGTCCGCTTTACCGGAGTGCTAACTCTATACG
AGAGGCTAGTACCATTGACACACTATTACGAGTGTCCTTATGTACAGCTGTGACCCTTTA
TACAAGTGGGCAGAGCTGCGAGCTACGTCATCGGTCATAGAAATCGTATAAAAAAATGTA
TGAAAGAAATATTTTTAAAAATTGAAATTAACTATGGGTAAAATATTGGCACG

Reference(s)

Hozumi et al. (2013). Germline transgenesis of the chordate Ciona intestinalis with hyperactive variants of sleeping beauty transposable element. Dev. Dyn. 242(1):30-43

Sasakura et al. (2005). Transposon-mediated insertional mutagenesis revealed the functions of animal cellulose synthase in the ascidian Ciona intestinalis. Proc. Natl. Acad. Sci. U.S.A. 102(42):15134-9

Awazu et al. (2004). An enhancer trap in the ascidian Ciona intestinalis identifies enhancers of its Musashi orthologous gene. Dev. Biol. 275(2):459-72

Yoshida et al. (2017). Hox-mediated endodermal identity patterns pharyngeal muscle formation in the chordate pharynx. Development 144(9):1629-1634

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