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CIS-REGULATION

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  1. Clone 'AHC0AAA108YG21' > 
  2. Clone 'ciad066n05' > 
  3. Region 'REG00001161' > 
  4. Clone 'AHC0AAA201YN02' > 
  5. Region 'REG00001106'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C5:4201966-4203978|cdc25_µGATA BS12

Short name

Ci-cdc25(-2010/+3)_µGATA BS12

Region ID

REG00001106

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Yosuke Ogura (2017-01-30)

Annotator

Marion Gueroult-Bellone (2017-01-30)

Curator

Delphine Dauga (2017-05-30)

View position in WashU

Description

This sequence contains the short (27pb) cis-regulatory module CRM1 containing two GATA binding sites (as defined by Ogura and Sasakura, 2016). In this variant, both GATA binding sites were mutated (replaced by AATA). Sequence of CRM1-GATA BS12 mutated : tattagaacgaatccagctcttattta

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C5:4201966-4203978 by substitution

Regulated gene(s)

KH2012:KH.C5.12 (CDC25A; CDC25B; CDC25C)

Construct(s) made to test this region

CONS00001074: pSP.Cirobu.REG.KH2012.C5:4201966-4203978(µGATA BS12):prCirobu.bpForkhead>Kaede

Show in situ data
Overview of functional TF binding sites in this region

4,201,9664,203,978

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [4,201,966 / 4,203,978] on scaffold KhC5

Both GATA binding sites (resp. located at bp1 and 17 from the 5' of the above sequence) were mutated : GATA was replaced by AATA.

Show sequence

Length: 2,013

catattaaagacaggtgctgaagattgaccacaagtgtataacacccgctagcttacaac
agagtaagctctcaagataccgtatgtctaaaacgcaattagctacttcggatgtcaaat
aacctgaaattctgcaaaagaaaacagtttgcttactcgatcggcgaagatactctgcta
atccacgagatatcggcttatgacaaacgcccataagttacatcgccgatatgacccaat
tccatcggcaatacgaccactatgttattgcggtctttactaagtcataatccaacttta
taacgcttgcgtcgcagaacaactgttgcaataagctccaatggccacaccatatgttac
gctcgaggtataggaggccttttgagtatatacccgacattctaatactactcactcaat
ataatacacaatacaacgtatgtatgtatgctgcatggtactctcttaatcaacacaata
aaatgacatatccaaaaccccaataatactatagccagctaactagtatacgactcttag
attctagttattgtgcaatgaaccgtctagtatcgtcagttaaacaaccatgtaatagca
tagtctctttctctcagtgctcgaggagcattccactttagttttaacgcactacaaact
actatacatacgcaatttctttcagttaccaatacgctatagtcgctatataacgtggta
gcctcacgctacagttatgtgctttagatgtgaatacatccgcctgtcgatactggccac
ttgtcctatatacattggatacggcttatcggcacaaagtaaacaataacaatgcgtagt
atgtggtctgcaatgcgctgctctgggtatactggaacggtactacattgttgtacagta
atgataaagttttacgtagaccagtaactacctagaggtagctgaataggtgaatactgt
ttgactcttatgcttctgtctagtaatatataagagccgaagtctgtctcgctgcttaat
acgcgctcaaactgaaatcccaggcaagatgtctacgattaggaagttgagaaaaaaggc
ccaagagctatgagctaattcccttgcattgtccggtttggacgctaattgatattctga
aggcagctcatgtaaactggtttacgaagcatcttacaatccggccaagctgggacacag
cgtgcgagtgactacctttacctgtgacgcgattaccaagcgagagattaacctttttcc
tttggcggtgagtcgaaaaggaaacataccgctgggctctcgctcggtagcgagtttgct
gctggcaagtaaatcctacgaccaacaatactagcgcaccttggcccggctggatgcagt
caggatgtctcggttggctgtttgtacgctatgcgggcactatggatgcacgcaagtgga
ttaacgggtgggattcttgcttaatatatccgaagttgggaactagttacggattaggca
gcgagtcggttctaatttttatagaaaagtttgctccgcggagatatttacgagaagcac
cacatatatattgacgacgtatttttgcttactactactgtcctactaacgttgcacgaa
aaacacacaactagtcttcctttaactatgcaggcagaacatgagtgaatactagattag
ttttaattaacgaatcggtcactatcctgcactgttgcatattctccaattacatggaat
attgctaataatgcatgtataccgaatcgatctactaacaaattgaactcagctggttgt
gcgctgcatgtgcagttgaatgaatggtgccctgcatcgaaccgcagctgctattttaag
agtgctggtgcagtcgcctttttctcgctttactacaggttcagtgtgttttggtcgtcc
actgataagtagcgaaggccgaagcgtcgcggttcgggccccgaggcacgcactattcta
gcgtcataaataagagctggattcgttctaata

Reference(s)

Ogura et al. (2016). Developmental Control of Cell-Cycle Compensation Provides a Switch for Patterned Mitosis at the Onset of Chordate Neurulation. Dev. Cell 37(2):148-61

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