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CIS-REGULATION

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  1. Clone 'ma314p21' > 
  2. Transcript 'Cisavi.CG.ENS81.R767...' > 
  3. Molecular Tool 'Pax6-MO (Imai 2009)' > 
  4. Transcript 'Harore.CG.MTP2014.S7...' > 
  5. Region 'REG00001244'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C9:5649375-5650489|DDR1; DDR2; MUSK

Short name

DDR1/2_CRM24_1115pb

Region ID

REG00001244

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

no_output

Author

Diana S. José-Edwards (2017-09-09)

Annotator

Marion Gueroult-Bellone (2017-09-09)

Curator

Marion Gueroult-Bellone (2018-05-09)

View position in WashU

Description

This 1kb region, located upstream of DDR1/DDR2/MUSK (KH.C9.371), is not able to drive notochord activity.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C9:5648367-5650489|DDR1; DDR2; MUSK by deletion

Regulated gene(s)

KH2012:KH.C9.371 (DDR1; DDR2)

Construct(s) made to test this region

CONS00001247: p.Cirobu.REG.KH2012.C9:5649375-5650489>LacZ

Show in situ data
Overview of functional TF binding sites in this region

5,649,3755,650,489

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [5,649,375 / 5,650,489] on scaffold KhC9

This sequence was retrieved using ANISEED Blast tool, thanks to the information provided in FigS2.a of Jose-Edwards et al. 2015.

Show sequence

Length: 1,115

taaacaggccttgacatgaagttggttatgtttatatgtggctattttgtcatgggctta
ggataaattcgattttccggagatacatatttttgtctcaaaatatccaacgcgttttcg
gctacaaagatcaaaccccgctgagctattgtgcctcacgtcatacgcagacgcggtaat
ctggtgttgcctttttctcgacgtggtttcgtctaattttgaagagaaggcaatatcggc
gctcctttagttctgatagcattgcggtgttgaacgctctgtcaataccaggagagagcg
ggcttgagtatgttagcggaatagatgctgacttagtcatatagtgctaacaaggctgaa
acggcgctcaagttattttgttttctcgtcgaagtatcaggtgttagtgtgcttacgtag
aagcggtatcttgtaaaatatcacgtgtcagattacagtcggatcgaatgttaaacagtg
atgtaatttctattttgtatctttgtagtttaggtctctcaaagttgtttactgtcaggt
cattttcgaatccacgcagcggaaccttgacaacaggttacagtttacgcgtgctcgtaa
agaactcgcaatttgcgcaagcgtaactgatcattacgtttgtttctccagtgcggcttg
atttagttggggccgcggcgagacaacgcttataatctgaacgtctgactgtcaattatt
aaaccgtagacgagttaggctgtgcctgggctttggattagaattggagttaaagaaact
gttccctttgggaaaacgtcaacgctgaaaagcttgtgcaaggttcaccatgaagtcgaa
gtcattaccaccgagtgatcgatcgagtattataattacgttgctgttctgtcttctgct
gtcagaggtacaaccgactacagcacaaatagatacaggtaagatggaaatacttttgtt
gattttattaaagttttggttgattttatttaaagttataatatgttatgttcataaaaa
cacgggttttcttggcggcttcaatacaaacatgaatatacatatatgcatgatgcaatt
ttcatctcgaacgttacggtttaaataccaaaacc

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

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