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CIS-REGULATION

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  1. Clone 'cien55258' > 
  2. Clone 'ciad067i18' > 
  3. Region 'REG00001344' > 
  4. EST 'BW224375' > 
  5. Region 'REG00001260'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C7:40900-40993_AP1mut|Noe2; Olm2A/B

Short name

Noe/Olm_CRM112_94pb_AP1mut

Region ID

REG00001260

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Diana S. José-Edwards (2017-09-10)

Annotator

Marion Gueroult-Bellone (2017-09-10)

Curator

Marion Gueroult-Bellone (2018-05-09)

View position in WashU

Description

Notochord activity of Noe2/Olm2A/B 94pb enhancer is reduced by the mutation of the AP1 putative binding site. Mesenchyme activity is also observed.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C7:40900-40993|Noe2; Olm2A/B by substitution

Regulated gene(s)

KH2012:KH.C7.500 (OLFM2; OLFML2A; OLFML2B)

Construct(s) made to test this region

CONS00001263: p.Cirobu.REG.KH2012.C7:40900-40993_AP1mut>LacZ

Show in situ data
Overview of functional TF binding sites in this region

40,90040,993

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 Fox n/a acaaata [40,934 / 40,941] First Fox putative binding site of Noe2/Olm2A/B 94pb enhancer. This putative binding site is deemed dispensable through truncations experiments and do not evidently contribute to the notochord activity of the enhancer.
2 Homeobox n/a atta [40,966 / 40,970] Homeodomain putative binding site of Noe2/Olm2A/B 94pb enhancer. It is required for notochord expression.
3 Fox n/a acaaaca [40,971 / 40,978] Second Fox putative binding site of Noe2/Olm2A/B 94pb enhancer. It is required for notochord expression.

Sequence

Aniseed Coordinates: [40,900 / 40,993] on scaffold KhC7

AP1 site is mutated. AP1 : TGAC replaced by TTTT

Show sequence

Length: 94


gaatgttacgcgttagaaagtcgaaacagggcgaacaaatactcttcggttcggcactga
tgcgaaattagacaaacagtctcgctcttttcgc

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

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