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CIS-REGULATION

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  1. Transcript 'Phmamm.CG.MTP2014.S3...' > 
  2. Transcript 'Haaura.CG.MTP2014.S7...' > 
  3. Region 'REG00001298'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.S654:26922-27123_μF|Tbx6.b

Short name

Tbx6.b_-189_μF

Region ID

REG00001298

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Izumi Oda-Ishii (2018-03-13)

Annotator

Marion Gueroult-Bellone (2018-03-13)

Curator

Marion Gueroult-Bellone ()

View position in WashU

Description

This substitution does not affect a lot the level of expression and the expression pattern in the posterior vegetal domain. New weak activity is observed in anterior vegetal domain.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.S654:26922-27123|Tbx6.b by substitution

Regulated gene(s)

KH2012:KH.S654.3 (TBX2; TBX4; TBX6)

Construct(s) made to test this region

CONS00001318: p.(Cirobu.REG.KH2012.S654:26922-27123_μF)x4>GFP

Show in situ data
Overview of functional TF binding sites in this region

26,92227,125

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 TCF/LEF n/a ctttg [26,981 / 26,986] One of the 2 Tcf7 binding sites located on the 189pb upstream enhancer of KH.S654.3 (Tbx6.b). When 1 or both of these TFBS are missing or mutated, the enhancer is inactive.
2 TCF/LEF n/a aaaac [27,076 / 27,081] One of the 2 Tcf7 binding sites located on the 189pb upstream enhancer of KH.S654.3 (Tbx6.b). When 1 or both of these TFBS are missing or mutated, the enhancer is inactive.

Sequence

Aniseed Coordinates: [26,922 / 27,125] on scaffold KhS654

Mutation of 15pb of the 200pb enhancer of Tbx6.b (KH.S645.3). Mutated sequence is shown in capital letters.

Show sequence

Length: 204


cggaatgaggtactgtgaaactaaaccTGCCCCGCGATAGAGactcgttttcgatgtcac
tttgatcttaacgagtcattaacaaggacaactgcttcctgataagactccgaactctcc
tgctgattcgtgtctagcgcatgcagaacactccaaaactcagtgtacgcataccgtctt
gcatgtggcgcgttggtttgataa

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

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