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CIS-REGULATION

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  1. Region 'REG00001325'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C10:2815582-2817555|Ubp4,Ubp8,Ubp11

Short name

n/a

Region ID

REG00001325

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(complex_region)

Author

Diana S. José-Edwards (2018-05-08)

Annotator

Marion Gueroult-Bellone (2018-05-08)

Curator

Marion Gueroult-Bellone (2018-05-08)

View position in WashU

Description

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.C10.320 (USP11; USP32; USP4)

Construct(s) made to test this region

CONS00001345: p.Cirobu.REG.KH2012.C10:2815582-2817555>LacZ

Show in situ data
Overview of functional TF binding sites in this region

2,815,5822,817,555

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 T-Box n/a gtgtcta [2,816,571 / 2,816,578] This Brachyury binding site is located in the notochord enhancer of C10.320.
2 bHLH n/a cagttg [2,816,618 / 2,816,624] This is one of the 2 E-box binding sites located in the notochord enhancer of C10.320.
3 bHLH n/a cagttg [2,816,637 / 2,816,643] This is one of the 2 E-box binding sites located in the notochord enhancer of C10.320. Its deletion or its mutation inhibits enhancer activity in the notochord.

Sequence

Aniseed Coordinates: [2,815,582 / 2,817,555] on scaffold KhC10

This 1974bp sequence, located in the downstream region of KH.C10.320, drives reporter gene expression in notochord. This sequence was retrieved using ANISEED Blast tool, thanks to the information provided in FigS1.b and TableS5 of Jose-Edwards et al. 2015.

Show sequence

Length: 1,974


tctggcttggtgatgtccatttctttactgtaatttatataagcacataaattgtactgt
agcctttgcatataatgtgatcttttagaagattgtatttgcatctactgtgtaggaact
tctgtgatattgtgttgtgaagactttttgcaaattaacctcgacctcttgctttttttc
tccaatctctgatcatctggttacagtattaaactttattcaaattatcaagtgttttgt
aaaagttgtgtggaatagtatgttgtaggaagaggtaaatgaatgaatgaatgtaacttt
atcctcgcgtggccggaaaacgacagtcgtcataacatgggtttaacacctcgtgccagc
ttacgagttaccatgtatgttactttgtgggtgattattttttgggcgtattttttggca
gttgtttgaggtatgtaaaatggacgaaaaccgtgcgacaatcaaaaatatttgtatgtt
cgtgaatgccaaaacacatttctagcaagaatataaagttcggtactattgtttttattt
attcttagtgggtttttatttattcttgtgggtgaggtccttgtcaaggacttgggattt
acgccagattttgctcaatacccggaaagccgattcaagtctttcatgcgagcggtgccg
cgacattccttctatttaccgttgttagttgtttaataaaccacgatcaaggtatttgga
tattatgtgctgaaggtatcccatcttttcccaccttactatattattaacatttatact
gaaaagcattttaaatattacaatttggaaatttcagtatgaaaagcatgacaattttaa
tttcagcacaagcgctttatgattcgttgtaattattatttcagtatccccgccttttcg
tgccgctggtctcgttttagaggcagcgcttttttcttgcgcaacgaaactgtttgacag
cctgttggagcctagttagttttcgccgtgtgtctagtcaccacagtgctgtttagttca
agtaagtttaagtaagcagttgacgtttaagtaagcagttgacgagctaatatagagtac
tgtgggggaagatgggacacctttagcacttaatatccaaatatcctgatcgtgttttaa
acaattaacgacagtctatgaaagtcgtagggatacggtttaataaatccttgaatgttc
tttgtttattaccaaatgggacgaaaaggtgtcccatcttcccccaccctactataccta
gaaaatgctggtttttgcgaaccgttaaaacagatgcaaactggccttgattggttttcc
tataaccagcgtgatatatcttccacattaactggatcaatgaaacaagttaattaatct
tcaagttccttagcaaacaagcttaccagtaacggtgtttggtttgtcgcggctcttgtt
taatatcgcataaatgttccaggaattgttactaaccgtacagtctcgatatcagtgcaa
attgtcacgcgaatattttcacgttaacccagttttgtgagttttttacttgaaaattat
tttaaagtaggaacgcgggattatttggttgtctagaccagtgctcttcgacttttaatg
ggttggtgaaccccttaagttgttgcgtcgaactcatgcacccctgattgctttaacgta
aaactaatgaagtttcacaattcccagtgatgcaagatataaaattcatataaaaaaatg
taggaaagtggctaaattggttaaaaaagtttaaaatgaaaaaatattgctgaaaattgt
cgaagtttgtgatgcacccttgtatacctttttgcactggtgcacccctggcatattttg
gtgcaccctagggtgcaccgtgcacaccggttgaagagcactggtctagactctagagtg
tcctatataacccgcgaattcgttacttatattgtatttgataacgcggtattt

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

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