Description

This 198bp sequence, located in the downstream region of KH.C10.320, drives reporter gene expression in notochord. It contains 2 E-box and 1 Brachyury putative binding sites.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C10:2816477-2816808|Ubp4,Ubp8,Ubp11 by deletion

Regulated gene(s)

KH2012:KH.C10.320 (USP11; USP32; USP4)

Construct(s) made to test this region

CONS00001351: p.Cirobu.REG.KH2012.C10:2816491-2816688>LacZ

Overview of functional TF binding sites in this region

2,816,4912,816,688

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment
1	T-Box	n/a	gtgtcta	[2,816,571 / 2,816,578]	This Brachyury binding site is located in the notochord enhancer of C10.320.
2	bHLH	n/a	cagttg	[2,816,618 / 2,816,624]	This is one of the 2 E-box binding sites located in the notochord enhancer of C10.320.
3	bHLH	n/a	cagttg	[2,816,637 / 2,816,643]	This is one of the 2 E-box binding sites located in the notochord enhancer of C10.320. Its deletion or its mutation inhibits enhancer activity in the notochord.

Sequence

Aniseed Coordinates: [2,816,491 / 2,816,688] on scaffold KhC10

This sequence was retrieved using ANISEED Blast tool, thanks to the information provided in FigS1.b and TableS5 of Jose-Edwards et al. 2015.

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730