Login
Help

CIS-REGULATION

Submit your Data

  1. Clone 'AHC0AAA8YG10' > 
  2. Transcript 'Cisavi.CG.ENS81.R5.1...' > 
  3. Clone 'cien208289' > 
  4. Clone 'cicl010c20' > 
  5. Region 'REG00001333'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C10:2816564-2816650|Ubp4,Ubp8,Ubp11

Short name

n/a

Region ID

REG00001333

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Diana S. José-Edwards (2018-05-08)

Annotator

Marion Gueroult-Bellone (2018-05-08)

Curator

Marion Gueroult-Bellone (2018-05-08)

View position in WashU

Description

This 87bp sequence, located in the downstream region of KH.C10.320, drives reporter gene expression in notochord, central nervous system and tail muscles. It contains 2 E-box and 1 Brachyury putative binding sites.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C10:2816491-2816688|Ubp4,Ubp8,Ubp11 by deletion

Regulated gene(s)

KH2012:KH.C10.320 (USP11; USP32; USP4)

Construct(s) made to test this region

CONS00001353: p.Cirobu.REG.KH2012.C10:2816564-2816650>LacZ

Show in situ data
Overview of functional TF binding sites in this region

2,816,5642,816,650

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 T-Box n/a gtgtcta [2,816,571 / 2,816,578] This Brachyury binding site is located in the notochord enhancer of C10.320.
2 bHLH n/a cagttg [2,816,618 / 2,816,624] This is one of the 2 E-box binding sites located in the notochord enhancer of C10.320.
3 bHLH n/a cagttg [2,816,637 / 2,816,643] This is one of the 2 E-box binding sites located in the notochord enhancer of C10.320. Its deletion or its mutation inhibits enhancer activity in the notochord.

Sequence

Aniseed Coordinates: [2,816,564 / 2,816,650] on scaffold KhC10

This sequence was retrieved using ANISEED Blast tool, thanks to the information provided in FigS1.b and TableS5 of Jose-Edwards et al. 2015.

Show sequence

Length: 87


tcgccgtgtgtctagtcaccacagtgctgtttagttcaagtaagtttaagtaagcagttg
acgtttaagtaagcagttgacgagcta

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Back to Top