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CIS-REGULATION

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  1. Clone 'cibd084p19' > 
  2. Clone 'citb13k7' > 
  3. Region 'REG00001385' > 
  4. Clone 'cien165920' > 
  5. Region 'REG00001357'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C12:712425-712552_Myb3Mut|CbpD,CbpE,CbpN

Short name

n/a

Region ID

REG00001357

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Diana S. José-Edwards (2018-05-09)

Annotator

Marion Gueroult-Bellone (2018-05-09)

Curator

Marion Gueroult-Bellone (2018-05-09)

View position in WashU

Description

This 128bp sequence, located in the downstream region of KH.C12.16, drives reporter gene expression in mesenchyme, tail muscles, central nervous system but not in notochord. It contains 3 Fox putative binding sites. Compared to the notochordd-active WT enhancer, it lacks the Myb#3 binding site.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C12:712425-712552|CbpD,CbpE,CbpN by deletion

Regulated gene(s)

KH2012:KH.C12.16 (CPD; CPE; CPN1)

Construct(s) made to test this region

CONS00001377: p.Cirobu.REG.KH2012.C12:712425-712552_Myb3Mut>lacZ

Show in situ data
Overview of functional TF binding sites in this region

712,425712,552

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 Fox n/a acaaaca [712,452 / 712,459] This is one of the 3 Fox binding sites located in the notochord-active enhancer of KH.C12.16.
2 Fox n/a acaaata [712,465 / 712,472] This is one of the 3 Fox binding sites located in the notochord-active enhancer of KH.C12.16.
3 Helix Turn Helix / Tryptophane clusters n/a acaata [712,480 / 712,486] This is one of the 3 Fox binding sites located in the notochord-active enhancer of KH.C12.16.

Sequence

Aniseed Coordinates: [712,425 / 712,552] on scaffold KhC12

The Myb#3 binding site is mutated. CAACGG replaced by CGCAGG. This sequence was retrieved using ANISEED Blast tool, thanks to the information provided in FigS1.e and TableS5 of Jose-Edwards et al. 2015.

Show sequence

Length: 128


tcacatatatgcgttgtgacgtcatatacaaacaacaagaacaaatagtgacgtaacaat
acaaatatcgagtaaattcgacgtcacggttgaaccTGCgtgtggataagttaaccatga
cgtcaccg

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

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