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CIS-REGULATION

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  1. Region 'REG00001544' > 
  2. Region 'REG00001375' > 
  3. Region 'REG00001366'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.L128:215034-215843|Furin,Nec1/2

Short name

n/a

Region ID

REG00001366

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

no_output

Author

Diana S. José-Edwards (2018-05-13)

Annotator

Marion Gueroult-Bellone (2018-05-13)

Curator

Marion Gueroult-Bellone (2018-05-13)

View position in WashU

Description

This 810bp sequence, located in an intronic region of KH.L128.2, drives no reporter gene expression in notochord, tail muscles, mesenchyme and central nervous system. Compared to the notochord-active WT enhancer, it lacks a cluster of 2 Myb#2, 8 Brachyury, 3 Fox, 4 E-box, 1 Myb#3 and 1 Sp1/Klf putative binding sites.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.L128:214285-215843|Furin,Nec1/2 by deletion

Regulated gene(s)

KH2012:KH.L128.2 (FURIN; PCSK1; PCSK2)

Construct(s) made to test this region

CONS00001386: p.Cirobu.REG.KH2012.L128:215034-215843>lacZ

Show in situ data
Overview of functional TF binding sites in this region

215,034215,843

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [215,034 / 215,843] on scaffold KhL128

This sequence was retrieved using ANISEED Blast tool, thanks to the information provided in FigS3.c and TableS5 of Jose-Edwards et al. 2015.

Show sequence

Length: 810

atacgtggtatcgggttaatacatgttggatgttataataatacgtttgtttctatttga
atcgggttgcttattaagcgttgtctaattaagcaacatatcgttgtataaagcccgcca
atagcctacaccacgcagatacagtcgtggtcgcttaaacaaatacgtcggtaaaatacg
ttgtttatatttttacctgaacatgctcaaggtatcgaacatggttatttccaccgtcac
aagcatcagtgtcaatagtgagctcaagcacgccatctgacggaatgtcaaacatctgct
caacgacaccagcttcgcatctgaaccggggtggaactgtcgtccaagttttcgccattt
ttacctaaagtaagtaagaacattaaatactgcaaatcatgcagcaagtctatagcgtgc
ctggatacatcagccataaagaatataatctgtacttattacagtaactataaggtaagc
ttagcttgcataactagaagctatttctaagtggttagagcgctctcagtataccaggtc
cgaaattcagtgccgctaacattgtgtgtgtttgttccttggtaataatgactgtcgttt
gaggatttgagaataaaaaatacattctttaagtttaaaccgttcattcgcccatcaaag
ctgtatattatagtttttagctaaaataacaaacaaatatataaagtagcctacctaagt
ttagctaacccatgtattcattcattacctttattttacatgaaatcattcatttattct
acattcgttcaactcatattatcgtttcaa

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

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