Aniseed - Cis-regulation

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C3.6290100-6290899

Short name

n/a

Region ID

REG00001480

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Alicia Madgwick (2019-01-08)

Annotator

Patrick Lemaire (2019-01-08)

Curator

Delphine Dauga (2019-01-17)

View position in WashU

Description

Hierarchy of Regulatory Regions

Cirobu.REG.KH2012.C3.6290100-6290899 REG00001480 [ inactive_region ]

Regulated gene(s)

KH2012:KH.C3.411 (BMP4; LEFTY1; LEFTY2)

Construct(s) made to test this region

CONS00001508: pCirobu.REG00001480:Ciinte.bpFog>NLSLacZ

Show in situ data

Overview of functional TF binding sites in this region

6,290,1006,290,899

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [6,290,100 / 6,290,899] on scaffold KhC3

Primer used for cloning : Forward : tacggcgagacccgct Reverse : tgtgctattacctattgtactgcg

Show sequence

Length: 800

TACGGCGAGACCCGCTgtggcacctgcaattcgcgccagagtatggcccctattacccca
acatatatagggccttcttattagaaatgcgtaatatgtaagttttaatttagtttaagc
gttaaagatggccgctatgagcaggtttaaaaggtcgttgttaattgttaagaactcgaa
tgtccttggcctaaaaagatcccatggattacatggtgacagcataagagcggttgtaag
attttaaattgtatattacgtcgtttgatgacacttagcgacgtttacatgacgtcacac
gtctagacgtagttaatcctcaagcgacaagtccggtcgatggtttgtccctccctgggt
ctcgtcttcgatgtgtattcggtcactaactctctaacagtgctcatagtgcttgctcgc
gatgcattattacgcagcaatcgtcattgcgttgcaacaatggaaaacaatggcgacatt
attcgttacgttcacgtaacaatgcggcggactctctaacgaaaatcgcgtctaatttta
cttgttaagcgcgtgaatgttttgtgacgaaacaatagaacgattatcttctattgtgac
atgacaatcgcaatttaaatcgtagaatcgcgctaattgtttattaatttacgaacaatg
aaaaccccgtttatacacctggatataatataatcatctaaattcgcgaaacttttactt
cccagagggtttatatcggaaataggcaaatattttttacaattaaaccacattgtCGCA
GTACAATAGGTAATAGCACA

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87