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  1. Region 'REG00001481'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C3.6291450-6291999

Short name

n/a

Region ID

REG00001481

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Alicia Madgwick (2019-01-08)

Annotator

Patrick Lemaire (2019-01-08)

Curator

Delphine Dauga (2019-01-17)

View position in WashU

Description

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.C3.411 (BMP4; LEFTY1; LEFTY2)

Construct(s) made to test this region

CONS00001510: pCirobu.REG00001481>NLSLacZ

Show in situ data
Overview of functional TF binding sites in this region

6,291,4506,291,999

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [6,291,450 / 6,291,999] on scaffold KhC3

Primer used for cloning : Forward : tgttaattgtttaaaacacgattaga Reverse : acagcgctgtggttcgag

Show sequence

Length: 550

TGTTAATTGTTTAAAACACGATTAGAatatttgggtattgtgtgctaaaggtgtcctatt
tttccctaccctactatacatattttcactttaaaccagaaatttattttgcatcttaaa
attgttctaaatatgaattatttgatcgttgtttctaattaatcccgaacaaagttgcgg
taattgttactgaaagaaatttgcgacaattcctttgtttggtgcgctcactttcgttga
ttgaacaataatagcggtgcaagctttctattgtctcatcctttgtgtctggcaactatc
gtcgtttatagcgttatctggtcggagttcgcaataacgacttttgtctggaacacccgc
ttcaaagtgacgaagacaaaaagaaaatgttattgtttctactttgagataagcagacga
ccactgccggtatataagtcgccgcatctcagggtgggattcattttactcaacaactcg
cagcgggtaaccggaagtgacaagaacgagatctagacttttacctcaagttCTCGAACC
ACAGCGCTGT

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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