Aniseed - Cis-regulation

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C10.3633300-3633899

Short name

n/a

Region ID

REG00001483

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Alicia Madgwick (2019-01-08)

Annotator

Patrick Lemaire (2019-01-08)

Curator

Delphine Dauga (2019-01-17)

View position in WashU

Description

Hierarchy of Regulatory Regions

Cirobu.REG.KH2012.C10.3633300-3633899 REG00001483 [ inactive_region ]

Regulated gene(s)

KH2012:KH.C10.338 (NKX2-1; NKX2-2; NKX2-4)

Construct(s) made to test this region

CONS00001511: pCirobu.REG00001483:Ciinte.bpFog>NLSLacZ

Show in situ data

Overview of functional TF binding sites in this region

3,633,3003,633,899

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [3,633,300 / 3,633,899] on scaffold KhC10

Primer used for cloning : Forward : tctaatttatttaggacaaacggg Reverse : tttaatctaattcattttgtcattttt

Show sequence

Length: 600

TCTAATTTATTTAGGACAAACGGGtaaatatgtttttgatgaacagtttggcaacaaaaa
tgattgccgcattgtgtttggttgaagggcaagcggcacaaacgttgccttatcgattgt
ttagcatttataggcggaaaatatcacagtgcatttcgcacgttgtataaagatatcggt
aaaatccacgctatagatccaataatcggaatgatgcaaccctgtatttcgctctgctga
acaaatcgcgtgtgtttttgcggattaacacgctttaatgttcgaataccaccgcgtaaa
agcgtttgggcaagcgactcggcgcggtaatgcgcgattgtgacgtaacaaacacctagc
gatgaacgcgaagcgctaatcctgacttgcaatccggtaaattaaatattggccgacccg
ttaattcgtatttcgttggattttgcgtcttaattatacatcgaatgattcgtaggatta
ttcactaaaatgggaatcctggttcaaatctaacgggatattgaatgcaaattttctttg
ctaactctacctgtttaaaacgccgcaaatcatAAAAATGACAAAATGAATTAGATTAAA

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87