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  1. Region 'REG00001496'

Cis-regulatory Region

Name

Cirobu.REG.KhC6.1500090-1502346|Neurogenin

Short name

n/a

Region ID

REG00001496

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Alberto Stolfi (2019-03-11)

Annotator

Marion Gueroult-Bellone (2019-03-11)

Curator

Marion Gueroult-Bellone (2019-03-11)

View position in WashU

Description

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.C6.129 (NEUROG1; NEUROG2; NEUROG3)

Construct(s) made to test this region

CONS00001534: p.Cirobu.REG.KhC6.1500090-1502346::bpFOG>H2B::mCherry

CONS00001529: p.Cirobu.REG.KhC6.1500090-1502346>H2B::mCherry

CONS00001533: p.Cirobu.REG.KhC6.1500090-1502346>Galnt7::CFP

CONS00001532: p.Cirobu.REG.KhC6.1500090-1502346>nls::LacZ

CONS00001531: p.Cirobu.REG.KhC6.1500090-1502346>H2B::VenusYFP

CONS00001530: p.Cirobu.REG.KhC6.1500090-1502346>H2B::Venus

CONS00001528: p.Cirobu.REG.KhC6.1500090-1502346>unc-76::venusYFP

CONS00001527: p.Cirobu.REG.KhC6.1500090-1502346>unc-76::mCherry

CONS00001526: p.Cirobu.REG.KhC6.1500090-1502346>unc-76::eGFP

CONS00001525: p.Cirobu.REG.KhC6.1500090-1502346>unc-76::venus

Show in situ data
Overview of functional TF binding sites in this region

1,500,0901,502,346

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,500,090 / 1,502,346] on scaffold KhC6

The Neurog b-line CRM was cloned using the following primers: Neurog −3,010 forward (5′-GTCTGTTTCCGCATACATGC-3′) and Neurog −773 reverse (5′-CTTATACGCCGAACCTCATG-3′). This enhancer is active in the bipolar tail neuron precursors.

Show sequence

Length: 2,257

gtctgtttccgcatacatgcaatatgcacgcgttcattataatatcaagttttaccaatt
tccgtaaatttttaatggaacagtgtaattaacccgactatattaccgttttccgatcgc
attgttacgtcacaataaaaccgcggtgtgttctgcgaagttaggaagtatctcattacg
tcataatcgtcggatagttttgattgttttatcataatgcggactaagtagtccattact
acaccgacgtaatcgccactttgtgacgtcacaataccgcaccagatcaatgacgtcatc
aaaggggaaactcggaagtaataagtgacgaaatcaaaggcggaattatgacgttttcgc
ttttgattgttacgtacgtgggaaagagcaaacgaatcgggaaacgttttgctatcgtag
attgtgacgaaataatgaaatagattgtgacgtcatgatgatttttatctccgaataaac
ggaatcgagttatcagcacacgcgtcacgaacattgtttaccaactgaatacaattgtta
agttatttaaataaattttaatcaccttaaaccgagctgaattacacgccgatcaaacat
atattatttgttttactttgtcgttacatttgtacttgctaaagcaaattcgctttaaaa
aacggcccttctgaaagctttgactggtaaatgacatcaaagctgcggcgtttaaaagct
gtttactgttcgttgtttaatcggtacagtgtttggtgtttattctatactccagtctgg
tttattttatttattttacaccgttttggttaattttgttgcgtaatttaaaatctggta
cactgtttgtcccgtatttccctacccgcattacgagacctgattttacgctgtgtgacg
tcacgccatatggcacaaaatgcaactttaaacgaataagcagcgtccacaaaatacata
tcgggtgtattgcgtcgcgctaattgctcagatttccggaagtataatatttcaaccaat
ctctgcttattaaaacgttagattgactaattagaaaccacgaataatgttaattgctcc
aacccagtggttcattaattatgggttgtctaaaataaatgttggccaacacatttcaga
aaattaagaattccaaagtgttaatcacccacaaagtaacatacgtagtagctcgtaagc
gggcacgaggtgtatgaaacagaacacccgtgttataacaactgccatcatagatatcta
aacatatgtttagatatctatgctgccattactccgcaatgcgcggataaataaattaca
ttcattcgtatttgcaaaaatggtgtttttatgtctgtacggtccgggcataaagacgac
agattgccacgcatgcagtcgggatcaattatttataaataatatgaattattcacgagg
aatggcgctttcctgagccccgtgatttaggaaaatttgagaaaaatcacaaaaataaat
tacgaagtcgaaaatggagaaattttgggtaatttttatcatgaaatatgacatgcgtcc
aaattcgtgtatagtaggttggggtaaagtggtccatgtttttattctatttgtcgtctc
atttagtagtaaacaaagaatattcacaaaattataactgtatccttgcgactctaaaca
agcgttgttggttgttacaaaacgacctggaattatgggatattttgtgctgtacccatc
ttaccccacgatgctataaattctgattaaaaagcgacaagtttgtcaatagtaatttgg
agcttataattgacgaaacaaagtaatggttcggtttataaatgaaaataattgaaatat
atactggaacattctttatcgattaggagaatgacttttgaaaaatataaataatttctt
tataaaatcgggttggaaaatagaaataaaataagacagtaatgtgccggtatacatttt
ttctatattgattaaaattgaaacgtaaaataaaattgtcaaaacgcaaagtggaataca
gcgtaagacaataaatgtaattggacagttacggcttagtttaattgccataattttgtt
tactaccaaatggaacggtaaagtagaataaagtcctggaccatcttacccaacctatac
tgtatttaaagacgacgtaaattggcatgtagtcgcatttaaattctgagcaatattttg
tgtctctcggcagcgcgcatgaggttcggcgtataag

Reference(s)

Stolfi et al. (2015). Migratory neuronal progenitors arise from the neural plate borders in tunicates. Nature 527(7578):371-4

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