Aniseed - Cis-regulation

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S229:75185-75749|Neurogenin

Short name

n/a

Region ID

REG00001461

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

regulatory_region
(enhancer)

Author

Damien Gailly (2018-05-30)

Annotator

Marion Gueroult-Bellone (2018-05-30)

Curator

Marion Gueroult-Bellone ()

View position in WashU

Description

This 565bp sequence, located upstream of Neurogenin (S229_g05747), is active.

Hierarchy of Regulatory Regions

Phmamm.REG.CG.MTP2014.S229:75185-75749|Neurogenin REG00001461 [ regulatory_region (enhancer) ]

Regulated gene(s)

Phmamm.CG.MTP2014.S229.g05747 (NEUROG1; NEUROG2; NEUROG3)

Construct(s) made to test this region

CONS00001483: p.Phmamm.REG.CG.MTP2014.S229:75185-75749>LacZ

Show in situ data

Overview of functional TF binding sites in this region

75,18575,749

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [75,185 / 75,749] on scaffold S229

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : tcgttattttcccacgacaa Reverse : tctgttctgttcggtactttgc

Show sequence

Length: 565

tcgttattttcccacgacaagttcacgttcacgtacacaagaatgtcgcgacaaatcagg
cggaagaaacgggacaggagagccgaaaagtttgcgacgcgcgactccaagttcgaaaac
tattcaggattggcgtgtggcctaaagacgacggtcgcgcgaccacgtcgggctctgttg
gaaggaaaaattcggattcggaactggatttttgctttgtttttctgtttgctctctcgc
accaagaagagagaagcgcgcggaaaaaaagcgcgatcgcggccgattggtctttttctc
ttcaccgatgcttcgaatcgctttctctttttttatcgcgccgacgcgaatcggtttaaa
tagcgatgtcgctgcattttattctacattttcatttgcattcgaaacaacatcagcagt
tgcaaaccttcgtaccaagcgttggttatttcgagtgcaagaaaataaaaatttcgtgca
gagtttaagtctactaagattgtaagagcaacagagattttacgaaaaatgtttaaataa
attgcaaagtaccgaacagaacaga

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87