Login
Help

Submit your Data

  1. Region 'REG00001536'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C12:1333915-1334401|Rx

Short name

F0.49_Rx-517/-31

Region ID

REG00001536

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

D Aniello Enrico (2022-10-10)

Annotator

Marion Gueroult-Bellone (2022-10-10)

Curator

Marion Gueroult-Bellone (2022-10-10)

View position in WashU

Description

This 487bp cis-regulatory element is located upstream (-517/-31) of Rx TSS. It drives reporter gene activity in the central nervous system of Ciona robusta tailbuds and larvae, and ectopically in the mesenchyme.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C12:1333114-1334401|Rx by deletion

Regulated gene(s)

KH2012:KH.C12.152 (PAX6; RAX; RAX2)

Construct(s) made to test this region

CONS00001610: p.Cirobu.REG.KH2012.C12:1333915-1334401>LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,333,9151,334,401

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,333,915 / 1,334,401] on scaffold KhC12

This 487bp DNA fragment was amplified using the following primers : F 0.49 up: ATGTCGAAGTATTATAACGAG and F 0.49 down: CTAAATAACGAAATATGAATTAAAATC

Show sequence

Length: 487

ATGTCGAAGTATTATAACGAGTAATTTAACATAATTAATAGAAGAGACATAGCAGTGCAT
TTTCAAGTAGAATCAATACTAGCATTACCATCTGGCGATACACGGCCATATATACTCGGC
CCGGCTGCTATAATTTTTCAATAATTCCTGCTCACACGTCGTAATTCGTACTAAACAGAT
TGCTCTTTGTGATTTTCAATCACTTCCAAATCTTTGCTTACTCTTTAAACTAGGAGCGAA
GAAAGGAAAAAGGCAATTTGTTCCAGAACCTGGTTAAAACACAGTGCTTATGACGACATA
GCTGTAATACTGAAGTGATTGTTCGGTAAGAAAGAAGTGAACTCAAAGCGGACATTTTAC
AGTAAGGCTTAGAAAGTCAAGTACAGTATATTTAATAATGCATTCTAAAGCATATTTCAT
GGACACTTCATTCATTTTATTATTATAAAAAAATTAATTAGATTTTAATTCATATTTCGT
TATTTAG

Reference(s)

D'Aniello et al. (2011). Onecut is a direct neural-specific transcriptional activator of Rx in Ciona intestinalis. Dev. Biol. 355(2):358-71

Back to Top





By clicking "SEND", you accept the terms of our Privacy Policy.

You may choose to prevent this website from aggregating and analyzing the actions you take here. Doing so will protect your privacy, but will also prevent the owner from learning from your actions and creating a better experience for you and other users.