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CIS-REGULATION

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  1. Region 'REG00000904' > 
  2. Region 'REG00000916' > 
  3. Region 'REG00000908' > 
  4. Transcript 'KH2012:KH.C8.272.v6....' > 
  5. Region 'REG00001537'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C12:1333586-1333723|Rx

Short name

GO.14_Rx-897/-708

Region ID

REG00001537

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

D Aniello Enrico (2022-10-10)

Annotator

Marion Gueroult-Bellone (2022-10-10)

Curator

Marion Gueroult-Bellone (2022-10-10)

View position in WashU

Description

This 138bp element is located upstream (-897/-708) of Rx TSS. Cloned upstream the basal promoter of the human β-globin and Lac Z reporter gene, it has no enhancer activity.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C12:1333534-1334025|Rx by deletion

Regulated gene(s)

KH2012:KH.C12.152 (PAX6; RAX; RAX2)

Construct(s) made to test this region

CONS00001611: p.Cirobu.REG.KH2012.C12:1333586-1333723>LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,333,5861,333,723

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,333,586 / 1,333,723] on scaffold KhC12

This 138bp genomic DNA fragment was amplified using the following primers : G 0.14 up: GTTACTGTCTGAAACATCTGCTG and G 0.14 down: CATCGGTAAAGTATGGTACCG

Show sequence

Length: 138

GTTACTGTCTGAAACATCTGCTGATCATGAGTTTATTATCGACGTATTGTTAAACTAAAA
CATAGTCAAACGCAAAGTCTTAATCGTAACACATTTATTTTAGAATATTATAAAAAACGG
TACCATACTTTACCGATG

Reference(s)

D'Aniello et al. (2011). Onecut is a direct neural-specific transcriptional activator of Rx in Ciona intestinalis. Dev. Biol. 355(2):358-71

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