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CIS-REGULATION

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  1. Region 'REG00001544'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C12:1333829-1333859_CloxMUT|Rx

Short name

J-A-MUT_Rx-604/-573

Region ID

REG00001544

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

D Aniello Enrico (2022-10-10)

Annotator

Marion Gueroult-Bellone (2022-10-10)

Curator

Marion Gueroult-Bellone (2022-10-10)

View position in WashU

Description

The conserved putative Clox binding site of the J-A enhancer (located -604/-573bp upstream of Rx TSS) was mutated. This element is no longer able to drive reporter gene activity.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C12:1333829-1333859|Rx by substitution

Regulated gene(s)

KH2012:KH.C12.152 (PAX6; RAX; RAX2)

Construct(s) made to test this region

CONS00001618: p.Cirobu.REG.KH2012.C12:1333829-1333859_CloxMUT>LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,333,8291,333,859

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,333,829 / 1,333,859] on scaffold KhC12

This DNA element was prepared using a pair of synthetic purified oligonucleotides, containing the flanking KpnI and XhoI restriction sites at the 5′ and 3′ end, respectively, and replacing the core sequence of the Clox site with a sequence with a reduced binding affinity (small letters).

Show sequence

Length: 31

ATCGCGTTTTCATTcTccCcAcAATACCCTT

Reference(s)

D'Aniello et al. (2011). Onecut is a direct neural-specific transcriptional activator of Rx in Ciona intestinalis. Dev. Biol. 355(2):358-71

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