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CIS-REGULATION

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  1. Clone 'cima877135' > 
  2. Transcript 'KH2012:KH.C3.339.v1....' > 
  3. Transcript 'KH2012:KH.C3.339.v1....' > 
  4. Transcript 'KH2012:KH.C3.339.v1....' > 
  5. Region 'REG00001551'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C10:3636215-3638397|Titf

Short name

Titf1 2kb upstream (Yoshida 2017)

Region ID

REG00001551

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(complex_region)

Author

Yoshida Keita (2022-11-08)

Annotator

Marion Gueroult-Bellone (2022-11-08)

Curator

Marion Gueroult-Bellone (2022-11-08)

View position in WashU

Description

This Titf1 regulatory sequence is active in the endoderm and a small subset of neural cells.

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.C10.338 (NKX2-1; NKX2-2; NKX2-4)

Construct(s) made to test this region
n/a
Overview of functional TF binding sites in this region

3,636,2153,638,396

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [3,636,215 / 3,638,396] on scaffold KhC10

This 2182bp cis-regulatory sequence was amplified by polymerase chain reaction (PCR) using following primer pairs (F: 5- CGACTCTAGAGGATCCTAGTTCATGGTTAGCAATGAC-3; R: 5- GGCCGCAAGGGGATCCTCACAGCAAAGTTTCCAGTG-3)

Show sequence

Length: 2,182

ATCTCACAGCAAAGTTTCCAGTGTCGGAATAAATTATTAACTTGCAGGATTAAATATTAA
TTGACTTAAAACTGCTTCTGTTGACGCAGGCCAAGAATGTTACCGGCACTTGTGAGCTAA
GTATATTTCCACTCGTTAGCCTAGTGGAGATATCTGAACAATGCGTATGAGATCTGATCT
CTCCACTCTTGACTTGATAATGCTTCAGAGCGATTTGAAGATCTGGTTCCCTGCGTCTGC
GGGCCATCTCGGTCGCCGGAAAACGAGAGAGGCGGGGAGAAAAAGGCGCAGGTACGCGCC
GAAAATAGGAGAGAGATATTCATTGTTCCGCACAAAACCATTCGCGGATTATCTTTCCCT
ATGCCATCGTGAACAAACAGGTTATTATGATGTCACAGTTAAAAAATACTAACACATAAT
GCTCGGAAAGAAACTTTACTCCAGAATTTTTAAAATTCTTAGCACTAAATGTTTATATTT
TACCCTGCAGGCCAAACTTGTTAAATTTGCTGTTAAAACATATACTTAAACTGCTATATT
TTAAACTTTATTTTAGTCGCGGAACAATTAAACTTGTGACTTCACAGACTGTGGTCGACA
GCCACATCGAGCGGGAAATGAAACGGTTCGACGAACGGAAATCAAATGCAAATAAGAATC
GGAACTTTCCGTTCCAAACTAAATGCGACATTACAAACTCATTAGCGCTGATAAGCGTTT
GAAGTTTTTTCGACAGATGTATCTTGGCAGACACAAACAACCAGGAAGAGATAGACATCA
AACTCACACAGGATAGAGTATACGCATTGTATACGGAAATTTGATTCGTAAAAAACGACA
TTTTATTGTGAAAAATGACATTTTGGTTGAGGAAAATGATATTTTATCTGGAAAACTATA
GACTATTTCAAAAAAAACAAATTTATTCTGGATAACGACATTCCTTCTGGAAAATAAGAA
GTTCAGTTCCAGCATGCGGAAGCTGCTCTGGTTCTGGAAATAATTTTTTGTGTTAAAGGA
GCGAATTCTGAAAGGCTTAAAGCTTTGTTCGCAATGGGAGCTTAAAACAAACACAGTTAG
GAAAATATCCGGCGGCAGCTGTAGCTTCGCATTCCGCGAACTATCAAACAATTATTCCCG
AAATCATCTGTCGCAGAAGAACTTTGAAAATGTTCCGTCAGTTCCCGGGCTCGGGTTCAG
CGAGAAGAGCACTTGAGCCCCATCGAGCGGTGGTACGACGACGGCCGGGTGCTCAGTATA
TCGACTGGAGTTGGCACTCGAAAGTACGGGACACTTGAACGGCAGCTGAAGCTAGCATTA
GCGCGGGCTGCTATTGTGAGACGTAGGCCGCTATGTTGGATCCGCATTCGAACTAAATTA
GCTTGTGCTAATTTGTGAACAGTGTACAGCGTTCTCTAGCAACGAAACTGAGGTTTGCGT
CATTGTAAGGCCGCTGAACAAAGAAAGAAACAAAAAAGACCCAAAGCCACACATACTTTC
AACACTTCAAAATAAAAGCACAGCCACGCATTACGAAAAAATCAATCATTTGAAAGTAAA
GTTCCAAAATATGTTTTAGCGCACTGGAAAGTTGTGTCGCTAACACCATGCTAACACCTC
CTAAACGATATAAAATACTGTGGGTGAAGATGGATATCGTGATCAGATAATATCTCATGT
TTCCTAATCGTGTTTTTACCAAAAAACAACGCTATTTTAGAGTTGTGAGGATACGATTAC
ACATTCCTGAAAGTATTTTTTGTTTACCACCAAACGGGACGATAAATTCTAATAAAAACA
TCGGCGATCTTACCTAAATCTACTACATAAAAACCGACAGAGAAGATACAAGCCCTAATC
TGACTTGGCTCATGGAATAACAGCAATCTATTTGGTATTACACCAAAAACGGGATTTAAA
AAATCTCATGCAAAATGTGGTTATTTTGGTTTATCGAGGTTTTAGACGGCCGCCACCGGA
GTACCATTGTCTCGTATGACGTCAGAGATGACGTCACTGATTATTCTGAATCACGACCCT
TATTGTGTATCATGCCATGAGTCATTGCTAACCATGAACTAGCAAAGACGAACAGAATTG
AAAGAAAATTATCGTGAACAAAATGGCGGAATCGTTAATCGTAAAAAGTGACAAGGTTGT
TCTAATATAGTGCTGCGGTGTA

Reference(s)

Yoshida et al. (2017). Hox-mediated endodermal identity patterns pharyngeal muscle formation in the chordate pharynx. Development 144(9):1629-1634

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