Description

When compared to the -343bp cis-regulatory region, the -286bp element has a decreased activity in anterior neural a6.5 cells but a similar activity in vegetal cells.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.Kh2012.S544:4780-5236|Dmrt1 by deletion

Regulated gene(s)

KH2012:KH.S544.3 (DMRT1; DMRTA1; DMRTA2)

Construct(s) made to test this region

CONS00001680: p.Cirobu.REG.Kh2012.S544:4837-5236>LacZ

Overview of functional TF binding sites in this region

4,8375,236

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [4,837 / 5,236] on scaffold KhS544

There are two TSSs in this Dmrt1 cis-regulatory element. The sequence is 400bp long from the first TSS and 286bp long from the second TSS of Dmrt1. This cis-regulatory element is active in anterior neural cells a6.5 and in vegetal cells.

Reference(s)

Tokuhiro et al. (2017). Differential gene expression along the animal-vegetal axis in the ascidian embryo is maintained by a dual functional protein Foxd. PLoS Genet. 13(5):e1006741