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  1. Clone 'AHC0AAA231YE04' > 
  2. EST 'AHC0AAA231YJ12_RM1' > 
  3. EST 'AHC0AAA230YG15_RM1' > 
  4. EST 'BW140679' > 
  5. Construct 'CONS00001474'

Construct

ID

CONS00001474

Name

p.Phmamm.REG.CG.MTP2014.S411:109256-109999>LacZ

Min Promoter

CibpFoxAa

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of FoxAa basal promoter and LacZ reporter gene.
The tested cis-regulatory region is not able to drive LacZ expression.

This construct is testing Regulatory Region

REG00001452: Phmamm.REG.CG.MTP2014.S411:109256-109999|ELK

Sequence

Show sequence

Length: 744

ACCCTTAAACGCCTCGAACTTCCCATAGAAATGACTGACGTGATATTTGAACAAGCGTTG
GCAGGAAACACAAAGTTGGGTATTTTTCGTAATCGTGTCAACAACAGCTTACGACAGCTG
GCGTTCCTCGTCCTTAGTTTTGACGCGCATGGCCATCCACACTCGTAGGAAAGGGCCTCT
AATTGAGACGTGGCCATTATCACCAGCAGCTACAATAGTAAGTCCTCCCCAGCGAAAAGG
AAATGAAATCATCAGCAGGGCTCCAACTGCTAAGAAAAGCAGCTGTAATCATCGAGTGCC
GAGCAGCATATCTATCCGTGAATGCCCTGCTGTCGACAGAAGCCGTCCGCCGGAAAAGGA
AAAAGGCGTTTAAAGAAACAAAGTTTCCTCCCGGTGCCTTTCTCGCTGGCGCGCGGGTCT
TTGTCCGCAGTTGCGTTCTCTGCTGATTTGTTTGCGCTTCGCCGCGTGTACTTCCGCTGT
TTCGACGGTCGGAGCTCGCTTGAAGGGCTTTCGTGTTGATCGGCAAAAGGAATATCGCCA
CAGACGTGCGAGTTTGTTTCATCTCCAATAGTTGGTAAGGCGTACGAGTTGCTAGTCGAA
AGTGAGTTGCCCAGTTACGGCACCGAAGACAGGCAGTCCGCCATAAAGACGGTAGCAGTT
TGGTTCAAGTTCTGACTTGCGTGTCGCCGTATCAAGTTCAGTAGCCAGACAGGAACGACT
AACAAACACAAAGACAGGCAGGAA

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

Show in situ data





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