Aniseed - Construct

Construct

ID

CONS00001437

Name

p.Phmamm.REG.CG.MTP2014.S50:172500-172959>LacZ

Min Promoter

CibpFoxAa

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of LacZ reporter gene.
The tested cis-regulatory region drives LacZ expression in endoderm, TLCs, mesenchyme, notochord, brain, TVCs, muscle, nerve cord, epidermis and sensory vesicle.

This construct is testing Regulatory Region

REG00001415: Phmamm.REG.CG.MTP2014.S50:172500-172959|FoxAa

Sequence

Show sequence

Length: 460

GTGTAACTTTCACCTTCTATTTCAGTTAGAACTGTGTCTTGCAAAATTTAGAATCCCATG
AAAAGTGTGAGGTTGCGATACGTTGATAGTACCTAGCTTATCGCAAGCTCGTGCGGGCAC
AGGCTTGATCAAGATGCATTACGTCACGCATCAAGACAGTGGCCCGCCAATAAGGGCGTT
TCTAAAGCATGCGGCCGCTGGTCAGACCTGGGCCAGCCAAGGCGCGAGATTGGACCGCAT
TGATCATCGAAGAGAAAAAAGAAAGGGACTGGACGGAACTGGTGTCCAATATAAACCACT
GAATGACCAGACGGAATCATTCCAGCCTTTGAGCTCATAGTAGTCATTTCAAAGCATCAG
CAATCAGTTGTGAAACGGTAGCAAAATTACTAGACGAAATACGAATAGCCGTTTATACCT
TATATAACTAAGGGTTAGCGAGTGTGCAATTTAAACGCGT

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

Show in situ data