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  1. Construct 'CONS00001432'

Construct

ID

CONS00001432

Name

p.Phmamm.REG.CG.MTP2014.S128:60456-61171>LacZ

Min Promoter

CibpFoxAa

Reporter

LacZ

Comment

The studied sequence was cloned in a psp1.72.RfB::LacZ plasmid, upstream of FoxAa basal promoter and LacZ reporter gene.
The tested cis-regulatory region drives LacZ expression in mesenchyme, notochord, brain and muscle.

This construct is testing Regulatory Region

REG00001410: Phmamm.REG.CG.MTP2014.S128:60456-61171|Fgf9/16/20

Sequence

Show sequence

Length: 716

ATGGCAAATTGGCGTAAATGCCTTTATGGAGGTTTTCCGGTGTCATGAAACCGCGCTTGG
TGCGAATATTAATTCGATGAACAGATGTTTCGAAGCGACGCCACTGGCCATAAAGATAGT
GCAGCGCGATGCCCCTGCGTCAGCAGTCGGCGCACTTTCGTACCAACACAAAACAGCAAA
TTACCAGTCTACGAGGCGCAAATAAGCTCCTAAACGGCGACAAATTAGTGACACCGCCCC
CCGGCTCATTCAATTCGCAGCGCGGTCATCGTGGCAACAGTTACTTTCTCGGGGCAGTCA
TATTTCGATAATTACAACAGATAAGTACAGGTGATTAGTTGCATTCGAGCGATGCATACA
CGCCACGCTCTTTTGTCTATGAAAAGCAATTAGTGTTCGAATTGGAGCATTGTTACCGCT
TAGGGCAAACCAACCACGCTCGTTTTCGCGCGATCACACCTCCAGCAAGCAGTTGGACAA
CATACCCGAGAATATTTCACCCCTGTCGCTCATTACGGACCCATTAACCGCCCGTGCGCT
CAAAACCGCTGCGTCTCTAAGCCAATCACCAACAGCGAAGGCAAGCTGGGCGTTTTCACA
CGTGTTCTTGGCGTCGCATCGCCGGGAGATCCAAACCCTAATGCCCAAGTAATGTTGGCA
CAAGCCGTTTTTTCGCGGTTGCTCGTAGCACGATAGTTTTGTTGTTCAGCAGCGTC

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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