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  1. Construct 'CONS00001390'

Construct

ID

CONS00001390

Name

p.Cirobu.REG.KH2012.L128:214285-215033_Bra4Mut>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in mesenchyme and central nervous system.

This construct is testing Regulatory Region

REG00001370: Cirobu.REG.KH2012.L128:214285-215033_Bra4Mut|Furin,Nec1/2

Sequence

Show sequence

Length: 749

TTTAAAAAAGCAAAATTGCTACACCTCACCACTTACTTTCTAAACCCTTGTGAGCTGTCG
TCGTCGTTTGGTCGTCGATTCAATAATATGGAATCAGTGCCAAACGGAGACGTCATATTT
ATGGTCAAGTCTCCACGGCGAGAACTTGCAATGGTCAGAAACGCCTTTGGAATATAAGAA
GTAAAATACACGCCCCACGTGGGGCTCGAACCCACGACCGTTGGATTAAGAGTCCAACGC
TCTACCGACTGAGCTAGCAGGGCGGTAGAGATGAACTGATTAAGGTATCCTGTTAGCACA
TGCAACTTCATTTGTGATTATAGCATTAGAACCACCGCCGCAACAGTGTTTCACAACAGA
AGCAGATGAAGCATTTTGTTTTTTCTTGTCAACACTGATCTTTAGTTGTTGATTACACTC
GCGCGTACTTTGCAAGCTTTTTTGGTAAGCTATTTGCTTGTGAAGCTGTAGCAATGTAGC
AGGACAAACTACTAAATGTGGAAAGTATAAAACTTAGTGAAACGAGTATTTTGTATATAT
TAATCTGGCATTTAAAAATCAGCGTTTATGTTAGTTTGTATAAACAGAACTGGCTCGAAT
TCAACATCAAAGCTATTATTGTTATTGGTATTATTGAAGGGGATGAAAAAAACGCCAGTG
TTTGGTGGATATACTGATGTGTGCCATGTACGCTGCCGTATTTTATATGTATTCGTTACT
TTATACAATGTGTTTTGATGTATGCATGT

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

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