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CONSTRUCT

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  1. Construct 'CONS00001338' > 
  2. Construct 'CONS00001386' > 
  3. Construct 'CONS00001334' > 
  4. Construct 'CONS00001394' > 
  5. Construct 'CONS00001391'

Construct

ID

CONS00001391

Name

p.Cirobu.REG.KH2012.L128:214487-215033>lacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord and central nervous system.

This construct is testing Regulatory Region

REG00001371: Cirobu.REG.KH2012.L128:214487-215033|Furin,Nec1/2

Sequence

Show sequence

Length: 547

GGCTCGAACCCACGACCGTTGGATTAAGAGTCCAACGCTCTACCGACTGAGCTAGCAGGG
CGGTAGAGATGAACTGATTAAGGTATCCTGTTAGCACATGCAACTTCATTTGTGATTATA
GCATTAGAACCACCGCCGCAACAGTGTTTCACAACAGAAGCAGATGAAGCATTTTGTTTT
TTCTTGTCAACACTGACACTTAGTTGTTGATTACACTCGCGCGTACTTTGCAAGCTTTTT
TGGTAAGCTATTTGCTTGTGAAGCTGTAGCAATGTAGCAGGACAAACTACTAAATGTGGA
AAGTATAAAACTTAGTGAAACGAGTATTTTGTATATATTAATCTGGCATTTAAAAATCAG
CGTTTATGTTAGTTTGTATAAACAGAACTGGCTCGAATTCAACATCAAAGCTATTATTGT
TATTGGTATTATTGAAGGGGATGAAAAAAACGCCAGTGTTTGGTGGATATACTGATGTGT
GCCATGTACGCTGCCGTATTTTATATGTATTCGTTACTTTATACAATGTGTTTTGATGTA
TGCATGT

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data