Aniseed - Construct

Construct

ID

CONS00001385

Name

p.Cirobu.REG.KH2012.L128:214285-215843>lacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord.

This construct is testing Regulatory Region

REG00001365: Cirobu.REG.KH2012.L128:214285-215843|Furin,Nec1/2

Sequence

Show sequence

Length: 1,559

TTTAAAAAAGCAAAATTGCTACACCTCACCACTTACTTTCTAAACCCTTGTGAGCTGTCG
TCGTCGTTTGGTCGTCGATTCAATAATATGGAATCAGTGCCAAACGGAGACGTCATATTT
ATGGTCAAGTCTCCACGGCGAGAACTTGCAATGGTCAGAAACGCCTTTGGAATATAAGAA
GTAAAATACACGCCCCACGTGGGGCTCGAACCCACGACCGTTGGATTAAGAGTCCAACGC
TCTACCGACTGAGCTAGCAGGGCGGTAGAGATGAACTGATTAAGGTATCCTGTTAGCACA
TGCAACTTCATTTGTGATTATAGCATTAGAACCACCGCCGCAACAGTGTTTCACAACAGA
AGCAGATGAAGCATTTTGTTTTTTCTTGTCAACACTGACACTTAGTTGTTGATTACACTC
GCGCGTACTTTGCAAGCTTTTTTGGTAAGCTATTTGCTTGTGAAGCTGTAGCAATGTAGC
AGGACAAACTACTAAATGTGGAAAGTATAAAACTTAGTGAAACGAGTATTTTGTATATAT
TAATCTGGCATTTAAAAATCAGCGTTTATGTTAGTTTGTATAAACAGAACTGGCTCGAAT
TCAACATCAAAGCTATTATTGTTATTGGTATTATTGAAGGGGATGAAAAAAACGCCAGTG
TTTGGTGGATATACTGATGTGTGCCATGTACGCTGCCGTATTTTATATGTATTCGTTACT
TTATACAATGTGTTTTGATGTATGCATGTATACGTGGTATCGGGTTAATACATGTTGGAT
GTTATAATAATACGTTTGTTTCTATTTGAATCGGGTTGCTTATTAAGCGTTGTCTAATTA
AGCAACATATCGTTGTATAAAGCCCGCCAATAGCCTACACCACGCAGATACAGTCGTGGT
CGCTTAAACAAATACGTCGGTAAAATACGTTGTTTATATTTTTACCTGAACATGCTCAAG
GTATCGAACATGGTTATTTCCACCGTCACAAGCATCAGTGTCAATAGTGAGCTCAAGCAC
GCCATCTGACGGAATGTCAAACATCTGCTCAACGACACCAGCTTCGCATCTGAACCGGGG
TGGAACTGTCGTCCAAGTTTTCGCCATTTTTACCTAAAGTAAGTAAGAACATTAAATACT
GCAAATCATGCAGCAAGTCTATAGCGTGCCTGGATACATCAGCCATAAAGAATATAATCT
GTACTTATTACAGTAACTATAAGGTAAGCTTAGCTTGCATAACTAGAAGCTATTTCTAAG
TGGTTAGAGCGCTCTCAGTATACCAGGTCCGAAATTCAGTGCCGCTAACATTGTGTGTGT
TTGTTCCTTGGTAATAATGACTGTCGTTTGAGGATTTGAGAATAAAAAATACATTCTTTA
AGTTTAAACCGTTCATTCGCCCATCAAAGCTGTATATTATAGTTTTTAGCTAAAATAACA
AACAAATATATAAAGTAGCCTACCTAAGTTTAGCTAACCCATGTATTCATTCATTACCTT
TATTTTACATGAAATCATTCATTTATTCTACATTCGTTCAACTCATATTATCGTTTCAA

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data