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CONSTRUCT

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  1. Region 'REG00001319' > 
  2. Transcript 'KH2012:KH.C6.202.v1....' > 
  3. Transcript 'KH2012:KH.C9.479.v1....' > 
  4. Clone 'cien196396' > 
  5. Construct 'CONS00001383'

Construct

ID

CONS00001383

Name

p.Cirobu.REG.KH2012.C1:9941405-9941487>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives no LacZ expression in notochord. Sequence of the primers used : Forward : CggatccTAGAGAATCCTAGATTGCAAGGCCTTTTG Reverse : ACGTCATccatggCAAGTATATCTGCGAGAAATAATCAG Lowercase letters correspond to BamHI/NcoI restriction site.

This construct is testing Regulatory Region

REG00001363: Cirobu.REG.KH2012.C1:9941405-9941487|C6ST-like7

Sequence

Show sequence

Length: 83

CTCTAGAGAATCCTAGATTGCAAGGCCTTTTGTAAATATTTAACGTCAGTAAATACCGTC
TGATTACTTCTCGCAGATACTTG

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data