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- Construct 'CONS00001361'
Construct
ID
CONS00001361
Name
p.Cirobu.REG.KH2012.C5:4471567-4472163>LacZ
Min Promoter
Reporter
Comment
Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord. Sequence of the primers used : Forward : TtctagaTGGCAGTCTCATACATTCTAATCTGG Reverse : AggatccACAGCATAGTCACAACGGCTTTC Lowercase letters correspond to XbaI/BamHI restriction site.
Show sequence
Length: 597
TGGCAGTCTCATACATTCTAATCTGGAATAGCGAGTTCTGAATATTGTATTGTGTATTCT
ACAGCAGTGGTGGTGAGATTTAAAGAGATTTTTAAGCAGTTCTATAATGTTTAAATGAGA
ATACTTTGTAAGCAAACCATTCATAACAACTTCTATTATATTGAGTTAAATTGCCAGGTA
TGTCTAAATTCAACAGCTTCGGTAATGGCGCATTCTTTGTCATAATTATACGGAGGCAGG
CTGGTGTACGAAGAAATTCCATTTCGACACGACATGTTGCGCTGTAGTGACTCAAGTTTC
AAAGTTATGATAAGCTTTAAGAAAAGTGTAGACAGGCGTACTGCTTAACAAAACATTTGC
AGACTATTAAAAATTATTTAAAGTATTAATAAGCGCTACTTTAATATTTTTGTATGTTTA
CCAGTAATGAGTTTCGTACAGAATACGTTGCGCCGTGAATTGAAATGTGTGTTAACTTAA
CCTAGATTGAATGCATTCCGTCTCGAGTACAAATGCCTTTCTGATTTGTTGTCTTGTTTG
TTTTGCGTCTCTCTCGCTCTTGTTCAATATTTAGAAAGCCGTTGTGACTATGCTGTG