Aniseed - Construct

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Construct 'CONS00001359'

Construct

ID

CONS00001359

Name

p.Cirobu.REG.KH2012.S345:68522-68746>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord and mesenchyme. Sequence of the primers used : Forward : TACAtctagaCTAGCCGGTGGATAGGCCGGGCC Reverse : TctcgagGGTGCCATCTAGTACCTGG Lowercase letters correspond to XhoI/XbaI restriction site.

This construct is testing Regulatory Region

REG00001339: Cirobu.REG.KH2012.S345:68522-68746|KH.S345.4

Sequence

Show sequence

Length: 226

ACTAGCCGGTGGATAGGCAGGGCCAAGTGCCTGGCCATCCTTGCAAGAAGGGCCACGTAC
TAGATGGCCGCCCGAAGGCCACCTCAAGCCATTATCAAATGGCAAAGGCTCTAAAGAGTG
GGGGCTACCTACCGTAAGCTGCATGGCGCCGGGGCAAAAGCGCACCAAAACAAACTAGCC
GGTGGATAGGCCGGGCCTGCAAGAAGGGCCAGGTACTAGATGGCAC

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data