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- Construct 'CONS00001358'
Construct
ID
CONS00001358
Name
p.Cirobu.REG.KH2012.S345:68541-68828>LacZ
Min Promoter
Reporter
Comment
Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives no LacZ expression in notochord. Sequence of the primers used : Forward : TTgcatgcCCGGGCCAAGTGCCTGG Reverse : CTTGGCTGTactCCATCTAGT Lowercase letters correspond to XhoI/XbaI and XhoI/SphI restriction sites respectively.
Show sequence
Length: 288
GGGCCAAGTGCCTGGCCATCCTTGCAAGAAGGGCCACGTACTAGATGGCCGCCCGAAGGC
CACCTCAAGCCATTATCAAATGGCAAAGGCTCTAAAGAGTGGGGGCTACCTACCGTAAGC
TGCATGGCGCCGGGGCAAAAGCGCACCAAAACAAACTAGCCGGTGGATAGGCCGGGCCTG
CAAGAAGGGCCAGGTACTAGATGGCCGCCCGAAGGCCACACAGCCAAGAACAAGTCATAA
GACGATTGAACGATGGCCAGGCACTAGGTGGCCGCCCGAAAGCCAAAC