Login
Help

CONSTRUCT

Submit your Data

  1. Construct 'CONS00001406' > 
  2. Construct 'CONS00001373' > 
  3. Construct 'CONS00001250' > 
  4. Construct 'CONS00001399' > 
  5. Construct 'CONS00001356'

Construct

ID

CONS00001356

Name

p.Cirobu.REG.KH2012.S345:68522-68829>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord. Sequence of the primers used : Forward : TACAtctagaCTAGCCGGTGGATAGGCCGGGCC Reverse : ACTActcgagGTTTGGCTTTCGGGCGGCCACC Lowercase letters correspond to XhoI/XbaI restriction site.

This construct is testing Regulatory Region

REG00001336: Cirobu.REG.KH2012.S345:68522-68829|KH.S345.4

Sequence

Show sequence

Length: 308

ACTAGCCGGTGGATAGGCAGGGCCAAGTGCCTGGCCATCCTTGCAAGAAGGGCCACGTAC
TAGATGGCCGCCCGAAGGCCACCTCAAGCCATTATCAAATGGCAAAGGCTCTAAAGAGTG
GGGGCTACCTACCGTAAGCTGCATGGCGCCGGGGCAAAAGCGCACCAAAACAAACTAGCC
GGTGGATAGGCCGGGCCTGCAAGAAGGGCCAGGTACTAGATGGCACGCCCGAAGGCCACA
CAGCCAAGAACAAGTCATAAGACGATTGAACGATGGCCAGGCACTAGGTGGCCGCCCGAA
AGCCAAAC

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data