Aniseed - Construct

Construct

ID

CONS00001348

Name

p.Cirobu.REG.KH2012.C10:2816957-2817555>lacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives no LacZ expression in notochord.

This construct is testing Regulatory Region

REG00001328: Cirobu.REG.KH2012.C10:2816957-2817555|Ubp4,Ubp8,Ubp11

Sequence

Show sequence

Length: 599

AAATACCGCGTTATCAAATACAATATAAGTAACGAATTCGCGGGTTATATAGGACACTCT
AGAGTCTAGACCAGTGCTCTTCAACCGGTGTGCACGGTGCACCCTAGGGTGCACCAAAAT
ATGCCAGGGGTGCACCAGTGCAAAAAGGTATACAAGGGTGCATCACAAACTTCGACAATT
TTCAGCAATATTTTTTCATTTTAAACTTTTTTAACCAATTTAGCCACTTTCCTACATTTT
TTTATATGAATTTTATATCTTGCATCACTGGGAATTGTGAAACTTCATTAGTTTTACGTT
AAAGCAATCAGGGGTGCATGAGTTCGACGCAACAACTTAAGGGGTTCACCAACCCATTAA
AAGTCGAAGAGCACTGGTCTAGACAACCAAATAATCCCGCGTTCCTACTTTAAAATAATT
TTCAAGTAAAAAACTCACAAAACTGGGTTAACGTGAAAATATTCGCGTGACAATTTGCAC
TGATATCGAGACTGTACGGTTAGTAACAATTCCTGGAACATTTATGCGATATTAAACAAG
AGCCGCGACAAACCAAACACCGTTACTGGTAAGCTTGTTTGCTAAGGAACTTGAAGATT

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data