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  1. Construct 'CONS00001345'

Construct

ID

CONS00001345

Name

p.Cirobu.REG.KH2012.C10:2815582-2817555>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord.

This construct is testing Regulatory Region

REG00001325: Cirobu.REG.KH2012.C10:2815582-2817555|Ubp4,Ubp8,Ubp11

Sequence

Show sequence

Length: 1,974

AAATACCGCGTTATCAAATACAATATAAGTAACGAATTCGCGGGTTATATAGGACACTCT
AGAGTCTAGACCAGTGCTCTTCAACCGGTGTGCACGGTGCACCCTAGGGTGCACCAAAAT
ATGCCAGGGGTGCACCAGTGCAAAAAGGTATACAAGGGTGCATCACAAACTTCGACAATT
TTCAGCAATATTTTTTCATTTTAAACTTTTTTAACCAATTTAGCCACTTTCCTACATTTT
TTTATATGAATTTTATATCTTGCATCACTGGGAATTGTGAAACTTCATTAGTTTTACGTT
AAAGCAATCAGGGGTGCATGAGTTCGACGCAACAACTTAAGGGGTTCACCAACCCATTAA
AAGTCGAAGAGCACTGGTCTAGACAACCAAATAATCCCGCGTTCCTACTTTAAAATAATT
TTCAAGTAAAAAACTCACAAAACTGGGTTAACGTGAAAATATTCGCGTGACAATTTGCAC
TGATATCGAGACTGTACGGTTAGTAACAATTCCTGGAACATTTATGCGATATTAAACAAG
AGCCGCGACAAACCAAACACCGTTACTGGTAAGCTTGTTTGCTAAGGAACTTGAAGATTA
ATTAACTTGTTTCATTGATCCAGTTAATGTGGAAGATATATCACGCTGGTTATAGGAAAA
CCAATCAAGGCCAGTTTGCATCTGTTTTAACGGTTCGCAAAAACCAGCATTTTCTAGGTA
TAGTAGGGTGGGGGAAGATGGGACACCTTTTCGTCCCATTTGGTAATAAACAAAGAACAT
TCAAGGATTTATTAAACCGTATCCCTACGACTTTCATAGACTGTCGTTAATTGTTTAAAA
CACGATCAGGATATTTGGATATTAAGTGCTAAAGGTGTCCCATCTTCCCCCACAGTACTC
TATATTAGCTCGTCAACTGCTTACTTAAACGTCAACTGCTTACTTAAACTTACTTGAACT
AAACAGCACTGTGGTGACTAGACACACGGCGAAAACTAACTAGGCTCCAACAGGCTGTCA
AACAGTTTCGTTGCGCAAGAAAAAAGCGCTGCCTCTAAAACGAGACCAGCGGCACGAAAA
GGCGGGGATACTGAAATAATAATTACAACGAATCATAAAGCGCTTGTGCTGAAATTAAAA
TTGTCATGCTTTTCATACTGAAATTTCCAAATTGTAATATTTAAAATGCTTTTCAGTATA
AATGTTAATAATATAGTAAGGTGGGAAAAGATGGGATACCTTCAGCACATAATATCCAAA
TACCTTGATCGTGGTTTATTAAACAACTAACAACGGTAAATAGAAGGAATGTCGCGGCAC
CGCTCGCATGAAAGACTTGAATCGGCTTTCCGGGTATTGAGCAAAATCTGGCGTAAATCC
CAAGTCCTTGACAAGGACCTCACCCACAAGAATAAATAAAAACCCACTAAGAATAAATAA
AAACAATAGTACCGAACTTTATATTCTTGCTAGAAATGTGTTTTGGCATTCACGAACATA
CAAATATTTTTGATTGTCGCACGGTTTTCGTCCATTTTACATACCTCAAACAACTGCCAA
AAAATACGCCCAAAAAATAATCACCCACAAAGTAACATACATGGTAACTCGTAAGCTGGC
ACGAGGTGTTAAACCCATGTTATGACGACTGTCGTTTTCCGGCCACGCGAGGATAAAGTT
ACATTCATTCATTCATTTACCTCTTCCTACAACATACTATTCCACACAACTTTTACAAAA
CACTTGATAATTTGAATAAAGTTTAATACTGTAACCAGATGATCAGAGATTGGAGAAAAA
AAGCAAGAGGTCGAGGTTAATTTGCAAAAAGTCTTCACAACACAATATCACAGAAGTTCC
TACACAGTAGATGCAAATACAATCTTCTAAAAGATCACATTATATGCAAAGGCTACAGTA
CAATTTATGTGCTTATATAAATTACAGTAAAGAAATGGACATCACCAAGCCAGA

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

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