Aniseed - Construct

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Construct 'CONS00001334'

Construct

ID

CONS00001334

Name

p.Cirobu.REG.KH2012.S610:12703-12901>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord and central nervous system. Sequence of the primers used : Forward : CtctagaCAAACATCTTGCACCTAAGTGCTG Reverse : AAccatggCGTAATTATAACCTGGCACGAGATG Lowercase letters correspond to XbaI/NcoI restriction site.

This construct is testing Regulatory Region

REG00001314: Cirobu.REG.KH2012.S610:12703-12901|Kelch-related protein

Sequence

Show sequence

Length: 199

ACAAACATCTTGCACCTAAGTGCTGAAGAACTCCCCTCTTTGTCAATTTCAAGACCTGAA
AACATTTTCGTCATTTATAGAAAGTAAAAAGCTACAACATGAGAGTGAATAAATGAAACT
TACTTATCCTCGGGTGGCGAGAAAACCCTAGTCGTTATAACACGGGTGTTCATACATCTC
GTGCCAGGTTATAATTACG

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data