Aniseed - Construct

Construct 'CONS00001327' >
Construct 'CONS00001330'

Construct

ID

CONS00001330

Name

p.Cirobu.REG.KH2012.L65:311070-311303_BraMut2>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in the mesenchyme and in the muscle, but not in the notochord.

This construct is testing Regulatory Region

REG00001310: Cirobu.REG.KH2012.L65:311070-311303_BraMut2|Dyrk2/3/4

Sequence

Show sequence

Length: 234

CGACTTACCCCAACCTACTATATTCTACAGTTTACCTTTTGCTTGAAACGTTAAGTTTTA
TTTACCAAAATAACACCAGGCGTGTACGCCGCCATGTGACTCTAAATATAATATCTACAG
ATGTTATATTTACACCGGTTTGTTAAATAGGTTGCGAAGCGAACTTACAAACGCATGTAA
GGCGTTGGTGTCGAGGCTGTTAAATTGAGATGTACCAGACACACACGAGATATC

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data