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- Construct 'CONS00001328'
Construct
ID
CONS00001328
Name
p.Cirobu.REG.KH2012.L65:311070-311303>LacZ
Min Promoter
Reporter
Comment
Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in the notochord. Sequence of the primers used : Forward : AtctagaCGACTTACCCCAACCTACTATATTC Reverse : AggatccGATATCTCGTGTGTGcCTGGTAC Lowercase letters correspond to XbaI/BamHI restriction site.
Show sequence
Length: 234
CGACTTACCCCAACCTACTATATTCTACAGTTTACCTTTTGCTTGAAACGTTAAGTTTTA
TTTACCAAAATAACACCAGGCGTGTACGCCGCCATGTGACTCTAAATATAACACCTACAG
ATGTTATATTTACACCGGTTTGTTAAATAGGTTGCGAAGCGAACTTACAAACGCATGTAA
GGCGTTGGTGTCGAGGCTGTTAAATTGAGATGTACCAGACACACACGAGATATC