- Construct 'CONS00001327'
Construct
ID
CONS00001327
Name
p.Cirobu.REG.KH2012.L65:310840-311092>LacZ
Min Promoter
Reporter
Comment
Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives no LacZ expression in the notochord. Sequence of the primers used : Forward : TtctagaGTACCAGgCACACACGAGATATC Reverse : AggatccGATTACAGAACCGAATCGTGTTCG Lowercase letters correspond to XbaI/BamHI restriction site.
Show sequence
Length: 253
GTACCAGACACACACGAGATATCTATCCCCTATCTTATCACAGTAAGAGCAGAACAGCGT
TCAACCTAACACACAGGACGCGTAACATCACAATAGGACATTCCCAACGCTGTTTAACAC
TGTATTGACAACAAGAGAAATCTAATACGAACCCACGTATACAAAACACATCTTTTTACC
GCTCTGACAATGCTCTTTATGACCTCATGTCATTATTGTCCCCTTCAAACGAACACGATT
CGGTTCTGTAATC