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CONSTRUCT

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  1. Construct 'CONS00000048'
  2. Construct 'CONS00000034'
  3. Construct 'CONS00000040'
  4. Construct 'CONS00000051'
  5. Construct 'CONS00000041'

Construct

ID

CONS00000041

Name

pCi-Sna -1700/-157 pBra Syn::NLS-LacZ

Min Promoter

pBra

Reporter

LacZ

Comment

This construct includes the sequences from -1,7kb to -157bp of the Ci-Sna 5 regulatory region attached to the heterologous Ci-Bra basal promoter, which include 68bp upstream of the Ci-Bra trancsribed region and 77bp of the 3 sequence that extend through the first 15 codons of the coding region. In this construct the Ci-Sna 5 DNA fragment is placed in the same (syn) orientation as the direction of Ci-Bra/LacZ transcription. This construct mediates expression in the primary muscle lineages, mesenchyme and (?) TVCs but is inactive in the secondary muscle lineage, CNS and notochord.

This construct is testing Regulatory Region

REG00000055: Ci-Sna -1700/-157

Reference(s)